Potency of umbilical cord blood-and Wharton's jelly-derived mesenchymal stem cells for scarless wound healing

被引：92

作者：

Doi, Hanako ^{[1
,2
]}

Kitajima, Yuriko ^{[1
,3
]}

Luo, Lan ^{[1
]}

Yan, Chan ^{[1
]}

Tateishi, Seiko ^{[3
]}

Ono, Yusuke ^{[1
]}

Urata, Yoshishige ^{[1
]}

Goto, Shinji ^{[1
]}

Mori, Ryoichi ^{[4
]}

Masuzaki, Hideaki ^{[3
]}

Shimokawa, Isao ^{[4
]}

Hirano, Akiyoshi ^{[2
]}

Li, Tao-Sheng ^{[1
]}

机构：

[1] Nagasaki Univ, Atom Bomb Dis Inst, Dept Stem Cell Biol, Nagasaki 8528523, Japan

[2] Nagasaki Univ, Grad Sch Biomed Sci, Dept Plast & Reconstruct Surg, Nagasaki 8528523, Japan

[3] Nagasaki Univ, Grad Sch Biomed Sci, Dept Obstet & Gynecol, Nagasaki 8528523, Japan

[4] Nagasaki Univ, Grad Sch Biomed Sci, Dept Pathol, Nagasaki 8528523, Japan

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

STROMAL CELLS; MYOFIBROBLAST; COLLAGEN; MARROW; REPAIR;

D O I：

10.1038/srep18844

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Postnatally, scars occur as a consequence of cutaneous wound healing. Scarless wound healing is highly desired for patients who have undergone surgery or trauma, especially to exposed areas. Based on the properties of mesenchymal stem cells (MSCs) for tissue repair and immunomodulation, we investigated the potential of MSCs for scarless wound healing. MSCs were expanded from umbilical cord blood (UCB-MSCs) and Wharton's jelly (WJ-MSCs) from healthy donors who underwent elective full-term pregnancy caesarean sections. UCB-MSCs expressed lower levels of the pre-inflammatory cytokines IL1A and IL1B, but higher levels of the extracellular matrix (ECM)-degradation enzymes MMP1 and PLAU compared with WJ-MSCs, suggesting that UCB-MSCs were more likely to favor scarless wound healing. However, we failed to find significant benefits for stem cell therapy in improving wound healing and reducing collagen deposition following the direct injection of 1.0 x 10(5) UCB-MSCs and WJ-MSCs into 5 mm fullthickness skin defect sites in nude mice. Interestingly, the implantation of UCB-MSCs tended to increase the expression of MMP2 and PLAU, two proteases involved in degradation of the extracellular matrix in the wound tissues. Based on our data, UCB-MSCs are more likely to be a favorable potential stem cell source for scarless wound healing, although a better experimental model is required for confirmation.

引用

页数：10

共 26 条

[1]

Phase I trial: the use of autologous cultured adipose-derived stroma/stem cells to treat patients with non-revascularizable critical limb ischemia [J].