Tau phosphorylation in cells transfected with wild-type or an Alzheimer's disease mutant Presenilin 1

被引：23

作者：

Irving, NG

Miller, CCJ

机构：

[1] INST PSYCHIAT, DEPT NEUROSCI, LONDON SE5 8AF, ENGLAND

[2] INST PSYCHIAT, DEPT NEUROL, LONDON SE5 8AF, ENGLAND

[3] INST PSYCHIAT, DEPT PSYCHOL, LONDON SE5 8AF, ENGLAND

来源：

NEUROSCIENCE LETTERS | 1997年 / 222卷 / 02期

基金：

英国惠康基金;

关键词：

presenilin; Alzheimer's disease; tau; glycogen synthase kinase-3 beta; paired helical filaments; phosphorylation;

D O I：

10.1016/S0304-3940(97)13343-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

We have studied the effect of overexpressing either wild-type or an Alzheimer's disease mutant Presenilin 1 (PS1) on tau phosphorylation in transfected Chinese hamster ovary (CHO) and COS cells. Tau transfected into these cells is predominantly non-phosphorylated at many PHF-tau sites but co-transfection with the tan kinase glycogen synthase kinase-3 beta (GSK-3 beta) induces phosphorylation that generates epitopes for several phosphorylation-dependent antibodies. Co-transfection of tau with either wild-type or mutant PS1 did not alter tau phosphorylation as detected by five different antibodies. Likewise, co-transfection of the PS1s did not influence GSK-3 beta-mediated tau phosphorylation. The implications of these results for the pathogenesis of Alzheimer's disease are discussed. (C) 1997 Elsevier Science Ireland Ltd.

引用

页码：71 / 74

页数：4

共 19 条

[1] OVEREXPRESSED TAU-PROTEIN IN CULTURED-CELLS IS PHOSPHORYLATED WITHOUT FORMATION OF PHF - IMPLICATION OF PHOSPHOPROTEIN PHOSPHATASE INVOLVEMENT [J].

BAUM, L ;

SEGER, R ;

WOODGETT, JR ;

KAWABATA, S ;

MARUYAMA, K ;

KOYAMA, M ;

SILVER, J ;

SAITOH, T .

MOLECULAR BRAIN RESEARCH, 1995, 34 (01) :1-17

[2] THE STRUCTURE OF THE PRESENILIN-1 (S182) GENE AND IDENTIFICATION OF 6 NOVEL MUTATIONS IN EARLY-ONSET AD FAMILIES [J].

CLARK, RF ;

HUTTON, M ;

FULDNER, RA ;

FROELICH, S ;

KARRAN, E ;

TALBOT, C ;

CROOK, R ;

LENDON, C ;

PRIHAR, G ;

HE, C ;

KORENBLAT, K ;

MARTINEZ, A ;

WRAGG, M ;

BUSFIELD, F ;

BEHRENS, MI ;

MYERS, A ;

NORTON, J ;

MORRIS, J ;

MEHTA, N ;

PEARSON, C ;

LINCOLN, S ;

BAKER, M ;

DUFF, K ;

ZEHR, C ;

PEREZTUR, J ;

HOULDEN, H ;

RUIZ, A ;

OSSA, J ;

LOPERA, F ;

ARCOS, M ;

MADRIGAL, L ;

COLLINGE, J ;

HUMPHREYS, C ;

ASHWORTH, A ;

SARNER, S ;

FOX, N ;

HARVEY, R ;

KENNEDY, A ;

ROQUES, P ;

CLINE, RT ;

PHILLIPS, CA ;

VENTER, JC ;

FORSELL, L ;

AXELMAN, K ;

LILIUS, L ;

JOHNSTON, J ;

COWBURN, R ;

VIITANEN, M ;

WINBLAD, B ;

KOSIK, K .

NATURE GENETICS, 1995, 11 (02) :219-222

[3] Increased amyloid-beta 42(43) in brains of mice expressing mutant presenilin 1 [J].

Duff, K ;

Eckman, C ;

Zehr, C ;

Yu, X ;

Prada, CM ;

Pereztur, J ;

Hutton, M ;

Buee, L ;

Harigaya, Y ;

Yager, D ;

Morgan, D ;

Gordon, MN ;

Holcomb, L ;

Refolo, L ;

Zenk, B ;

Hardy, J ;

Younkin, S .

NATURE, 1996, 383 (6602) :710-713

[4] MONOCLONAL-ANTIBODY AT8 RECOGNIZES TAU-PROTEIN PHOSPHORYLATED AT BOTH SERINE-202 AND THREONINE-205 [J].

GOEDERT, M ;

JAKES, R ;

VANMECHELEN, E .

NEUROSCIENCE LETTERS, 1995, 189 (03) :167-170

[5] EPITOPE MAPPING OF MONOCLONAL-ANTIBODIES TO THE PAIRED HELICAL FILAMENTS OF ALZHEIMERS-DISEASE - IDENTIFICATION OF PHOSPHORYLATION SITES IN TAU-PROTEIN [J].

GOEDERT, M ;

JAKES, R ;

CROWTHER, RA ;

COHEN, P ;

VANMECHELEN, E ;

VANDERMEEREN, M ;

CRAS, P .

BIOCHEMICAL JOURNAL, 1994, 301 :871-877

[6]

Guidato S, 1996, J NEUROCHEM, V66, P1698

[7] CHROMOSOME 14-ENCODED ALZHEIMERS-DISEASE - GENETIC AND CLINICOPATHOLOGICAL DESCRIPTION [J].

HALTIA, M ;

VIITANEN, M ;

SULKAVA, R ;

ALAHURULA, V ;

POYHONEN, M ;

GOLDFARB, L ;

BROWN, P ;

LEVY, E ;

HOULDEN, H ;

CROOK, R ;

GOATE, A ;

CLARK, R ;

KORENBLAT, K ;

PANDIT, S ;

KELLER, HD ;

LILIUS, L ;

LIU, L ;

AXELMAN, K ;

FORSELL, L ;

WINBLAD, B ;

LANNFELT, L ;

HARDY, J .

ANNALS OF NEUROLOGY, 1994, 36 (03) :362-367

[8] PHENOTYPE OF CHROMOSOME 14-LINKED FAMILIAL ALZHEIMERS-DISEASE IN A LARGE KINDRED [J].

LAMPE, TH ;

BIRD, TD ;

NOCHLIN, D ;

NEMENS, E ;

RISSE, SC ;

SUMI, SM ;

KOERKER, R ;

LEAIRD, B ;

WIER, M ;

RASKIND, MA .

ANNALS OF NEUROLOGY, 1994, 36 (03) :368-378

[9] ALZHEIMER'S DISEASE-LIKE PHOSPHORYLATION OF THE MICROTUBULE-ASSOCIATED PROTEIN TAU BY GLYCOGEN SYNTHASE KINASE-3 IN TRANSFECTED MAMMALIAN CELLS [J].

LOVESTONE, S ;

REYNOLDS, CH ;

LATIMER, D ;

DAVIS, DR ;

ANDERTON, BH ;

GALLO, JM ;

HANGER, D ;

MULOT, S ;

MARQUARDT, B ;

STABEL, S ;

WOODGETT, JR ;

MILLER, CCJ .

CURRENT BIOLOGY, 1994, 4 (12) :1077-1086

[10] Phosphorylation of tau by glycogen synthase kinase-3 beta in intact mammalian cells: The effects on the organization and stability of microtubules [J].

Lovestone, S ;

Hartley, CL ;

Pearce, J ;

Anderton, BH .

NEUROSCIENCE, 1996, 73 (04) :1145-1157

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