Fertilization-induced changes in the microtubular architecture of the maize egg cell and zygote-an immunocytochemical approach adapted to single cells

被引:21
作者
Hoshino, Y
Scholten, S
von Wiegen, P
Lörz, H
Kranz, E
机构
[1] Univ Hamburg, Biozentrum Klein Flottbek, D-22609 Hamburg, Germany
[2] Hokkaido Univ, Field Sci Ctr No Biosphere, Kita Ku, Sapporo, Hokkaido 0600811, Japan
[3] Univ Hamburg, Bot Garten, D-22609 Hamburg, Germany
来源
SEXUAL PLANT REPRODUCTION | 2004年 / 17卷 / 02期
关键词
anti-alpha-tubulin antibody; maize; microtubule; Zea mays; zygote development;
D O I
10.1007/s00497-004-0221-0
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
By using single cell micromanipulation techniques, we developed an immunocytochemical procedure to examine subcellular protein localization in isolated and cultured cells. Localization of microtubules was examined in isolated single egg cells and developing zygotes of maize with anti-alpha-tubulin antibodies. In egg cells, a few cortical microtubules were detected but well organized microtubules were rarely observed. In contrast, distinct cortical microtubules and strands of cytoplasmic microtubules radiating from the nucleus to the cell periphery were observed in developing zygotes. Solely cortical microtubules were observed in zygotes up to 7 h after in vitro fertilization. After this time, radiating microtubules additionally appeared, and persisted during zygote development. These results indicate early and pronounced fertilization-induced changes in microtubular organization in the fertilized egg cell of maize.
引用
收藏
页码:89 / 95
页数:7
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