Development and Application of a Novel Peptide Nucleic Acid Probe for the Specific Detection of Cronobacter Genomospecies (Enterobacter sakazakii) in Powdered Infant Formula

被引：41

作者：

Almeida, C. ^{[1
,2
]}

Azevedo, N. F. ^{[1
,2
]}

Iversen, C. ^{[3
,4
]}

Fanning, S. ^{[3
,4
]}

Keevil, C. W. ^{[2
]}

Vieira, M. J. ^{[1
]}

机构：

[1] Univ Minho, Ctr Engn Biol, IBB, P-4710057 Braga, Portugal

[2] Univ Southampton, Sch Biol Sci, Environm Healthcare Unit, Southampton SO16 7PX, Hants, England

[3] Univ Coll Dublin, UCD Vet Sci Ctr, Ctr Food Safety, Dublin 4, Ireland

[4] Univ Coll Dublin, UCD Vet Sci Ctr, Ctr Food Borne Zoon, Dublin 4, Ireland

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 09期

关键词：

IN-SITU HYBRIDIZATION; REAL-TIME PCR; DUBLINENSIS SP-NOV; PSEUDOMONAS-AERUGINOSA; STAPHYLOCOCCUS-AUREUS; RAPID IDENTIFICATION; ESCHERICHIA-COLI; BACILLUS-CEREUS; BLOOD CULTURES; MILK-PRODUCTS;

D O I：

10.1128/AEM.02470-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Here, we report a fluorescence in situ hybridization (FISH) method for rapid detection of Cronobacter strains in powdered infant formula (PIF) using a novel peptide nucleic acid (PNA) probe. Laboratory tests with several Enterobacteriaceae species showed that the specificity and sensitivity of the method were 100%. FISH using PNA could detect as few as 1 CFU per 10 g of Cronobacter in PIF after an 8-h enrichment step, even in a mixed population containing bacterial contaminants.

引用

页码：2925 / 2930

页数：6

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