Differentiation of human adult cardiac stem cells exposed to extremely low-frequency electromagnetic fields

被引：95

作者：

Gaetani, Roberto ^{[1
]}

Ledda, Mario ^{[2
]}

Barile, Lucio ^{[1
]}

Chimenti, Isotta ^{[1
]}

De Carlo, Flavia ^{[2
]}

Forte, Elvira ^{[1
]}

Ionta, Vittoria ^{[1
]}

Giuliani, Livio ^{[3
]}

D'Emilia, Enrico ^{[3
]}

Frati, Giacomo ^{[1
,4
,5
]}

Miraldi, Fabio ^{[6
]}

Pozzi, Deleana ^{[1
]}

Messina, Elisa ^{[1
]}

Grimaldi, Settimio ^{[2
]}

Giacomello, Alessandro ^{[1
]}

Lisi, Antonella ^{[2
]}

机构：

[1] Univ Roma La Sapienza, Cenci Bolognetti Fdn, Inst Pasteur, Dept Expt Med, I-00161 Rome, Italy

[2] CNR, Inst Neurobiol & Mol Med, I-00133 Rome, Italy

[3] ISPESL DIPIA, Rome, Italy

[4] Sapienza Univ Rome, Polo Pontino ICOT, Dept Cardiac Surg, I-04100 Latina, Italy

[5] IRCCS Neuromed, I-86077 Pozzilli, Italy

[6] Univ Roma La Sapienza, Dept Heart & Great Vessels Attilio Reale, I-00161 Rome, Italy

来源：

CARDIOVASCULAR RESEARCH | 2009年 / 82卷 / 03期

关键词：

Clinical translation protocols; ELF-EMF; Ca2+ ion cyclotron resonance; Cardiac regenerative medicine; MAGNETIC-FIELD; PROGENITOR CELLS; CALCIUM; LYMPHOCYTES; PROLIFERATION; CALMODULIN; MATURATION;

D O I：

10.1093/cvr/cvp067

中图分类号：

R5 [内科学];

学科分类号：

100201 [内科学];

摘要：

Modulation of cardiac stem cell (CSC) differentiation with minimal manipulation is one of the main goals of clinical applicability of cell therapy for heart failure. CSCs, obtained from human myocardial bioptic specimens and grown as cardiospheres (CSps) and cardiosphere-derived cells (CDCs), can engraft and partially regenerate the infarcted myocardium, as previously described. In this paper we assessed the hypothesis that exposure of CSps and CDCs to extremely low-frequency electromagnetic fields (ELF-EMFs), tuned at Ca2+ ion cyclotron energy resonance (Ca2+-ICR), may drive their differentiation towards a cardiac-specific phenotype. A significant increase in the expression of cardiac markers was observed after 5 days of exposure to Ca2+-ICR in both human CSps and CDCs, as evidenced at transcriptional, translational, and phenotypical levels. Ca2+ mobilization among intracellular storages was observed and confirmed by compartmentalized analysis of Ca2+ fluorescent probes. These results suggest that ELF-EMFs tuned at Ca2+-ICR could be used to drive cardiac-specific differentiation in adult cardiac progenitor cells without any pharmacological or genetic manipulation of the cells that will be used for therapeutic purposes.

引用

页码：411 / 420

页数：10

共 36 条

[1]

Antiarrhythmic engineering of skeletal myoblasts for cardiac transplantation [J].