Glycosylation engineering in Chinese hamster ovary cells using tricistronic vectors

被引：2

作者：

Dinter, A ^{[1
]}

Zeng, S ^{[1
]}

Berger, B ^{[1
]}

Berger, EG ^{[1
]}

机构：

[1] Univ Zurich, Inst Physiol, CH-8057 Zurich, Switzerland

来源：

BIOTECHNOLOGY LETTERS | 2000年 / 22卷 / 01期

关键词：

fucosyltransferase; N-acetylglucosaminyltransferase; P-selectin; PSGL-1; tricistronic vectors;

D O I：

10.1023/A:1005600222635

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Expression of recombinant glycoproteins carrying the sialyl-Lewis(X) epitope requires host cells equipped with appropriate glycosyltransferases. Chinese hamster ovary cells transfected with tricistronic vectors and encoding the resistance marker gene, neoR, in the third cistron and core 2 N-acetylglucosaminyltransferase or alpha 1,3-fucosyltransferase III or IV in either the first or second cistron, respectively, produced both enzyme activities in a constant ratio. A representative clone was transfected with PSGL-1 (P-selectin glycoprotien ligand 1) and conferred P-selectin-binding activity to PSGL-1.

引用

页码：25 / 30

页数：6

共 10 条

[1] DICISTRONIC TRANSCRIPTION UNITS FOR GENE-EXPRESSION IN MAMMALIAN-CELLS [J].

DIRKS, W ;

WIRTH, M ;

HAUSER, H .

GENE, 1993, 128 (02) :247-249

[2] Controlled proliferation by multigene metabolic engineering enhances the productivity of Chinese hamster ovary cells [J].

Fussenegger, M ;

Schlatter, S ;

Dätwyler, D ;

Mazur, X ;

Bailey, JE .

NATURE BIOTECHNOLOGY, 1998, 16 (05) :468-472

[3] In vivo specificity of human α1,3/4-fucosyltransferases III-VII in the biosynthesis of Lewisx and sialyl Lewisx motifs on complex-type N-glycans -: Coexpression studies from BHK-21 cells together with human β-trace protein [J].

Grabenhorst, E ;

Nimtz, M ;

Costa, J ;

Conradt, HS .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (47) :30985-30994

[4] CONSTRUCTION OF STABLE BHK-21-CELLS COEXPRESSING HUMAN SECRETORY GLYCOPROTEINS AND HUMAN GAL(BETA-1-4)GLCNAC-R ALPHA-2,6-SIALYLTRANSFERASE ALPHA-2,6-LINKED NEUAC IS PREFERENTIALLY ATTACHED TO THE GAL(BETA-1-4)GLCNAC(BETA-1-2)MAN(ALPHA-1-3)-BRANCH OF DIANTENNARY OLIGOSACCHARIDES FROM SECRETED RECOMBINANT BETA-TRACE PROTEIN [J].

GRABENHORST, E ;

HOFFMANN, A ;

NIMTZ, M ;

ZETTLMEISSL, G ;

CONRADT, HS .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 232 (03) :718-725

[5] THE NOVEL MECHANISM OF INITIATION OF PICORNAVIRUS RNA TRANSLATION [J].

JACKSON, RJ ;

HOWELL, MT ;

KAMINSKI, A .

TRENDS IN BIOCHEMICAL SCIENCES, 1990, 15 (12) :477-483

[6] Visualization of P-selectin glycoprotein ligand-1 as a highly extended molecule and mapping of protein epitopes for monoclonal antibodies [J].

Li, FG ;

Erickson, HP ;

James, JA ;

Moore, KL ;

Cummings, RD ;

McEver, RP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (11) :6342-6348

[7] Post-translational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P- and E-selectin [J].

Li, FG ;

Wilkins, PP ;

Crawley, S ;

Weinstein, J ;

Cummings, RD ;

McEver, RP .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (06) :3255-3264

[8]

Rees S, 1996, BIOTECHNIQUES, V20, P102

[9] EXPRESSION CLONING OF A FUNCTIONAL GLYCOPROTEIN LIGAND FOR P-SELECTIN [J].

SAKO, D ;

CHANG, XJ ;

BARONE, KM ;

VACHINO, G ;

WHITE, HM ;

SHAW, G ;

VELDMAN, GM ;

BEAN, KM ;

AHERN, TJ ;

FURIE, B ;

CUMMING, DA ;

LARSEN, GR .

CELL, 1993, 75 (06) :1179-1186

[10] TYROSINE SULFATION OF P-SELECTIN GLYCOPROTEIN LIGAND-1 IS REQUIRED FOR HIGH-AFFINITY BINDING TO P-SELECTIN [J].

WILKINS, PP ;

MOORE, KL ;

MCEVER, RP ;

CUMMINGS, RD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (39) :22677-22680

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