Molecular typing of haemorrhagic septicaemia-associated Pasteurella multocida isolates from Pakistan and Thailand using multilocus sequence typing and pulsed-field gel electrophoresis

被引：22

作者：

Moustafa, Ahmed M. ^{[1
]}

Bennett, Mark D. ^{[1
]}

Edwards, John ^{[1
]}

Azim, Kamran ^{[2
]}

Mesaik, Muhammed A. ^{[2
]}

Choudhary, M. Iqbal ^{[2
]}

Pathanasophon, Pornpen ^{[3
]}

Worarach, Apasara ^{[3
]}

Ali, Qurban ^{[4
]}

Abubakar, Muhammad ^{[4
]}

Anjum, Rehana ^{[4
]}

机构：

[1] Murdoch Univ, Sch Vet & Life Sci, Murdoch, WA 6150, Australia

[2] Univ Karachi, ICCBS, Karachi 75270, Pakistan

[3] Natl Inst Anim Hlth, Dept Livestock Dev, Bangkok 10900, Thailand

[4] NARC Colony, Natl Vet Labs, Islamabad 45500, Pakistan

来源：

RESEARCH IN VETERINARY SCIENCE | 2013年 / 95卷 / 03期

关键词：

Pasteurella multocida; Haemorrhagic septicaemia; Multilocus sequence typing; Pulsed field gel electrophoresis; Thailand; Pakistan; DISCRIMINATORY ABILITY; IDENTIFICATION; DIVERSITY;

D O I：

10.1016/j.rvsc.2013.07.003

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

A comparative genetic study of 23 field isolates. and vaccine strains of Pasteurella multocida associated with haemorrhagic septicaemia cases from Pakistan and Thailand was done using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The MLST sequence type (ST) for all 20 of the 23 isolates tested was 122. The PFGE results showed one band difference between the Pakistani and the Thai isolates. Sequence type 122 is the dominant associated profile with haemorrhagic septicaemia (HS) cases in South Asia. The study supports the concept of using PFGE for short-term epidemiology and MLST for long-term epidemiology. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：986 / 990

页数：5

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