Changes in soil microbial community structure and function in an alpine dry meadow following spring snow melt

被引:287
作者
Lipson, DA [1 ]
Schadt, CW
Schmidt, SK
机构
[1] San Diego State Univ, Dept Biol, San Diego, CA 92182 USA
[2] Univ Colorado, Dept Environm Populat & Organism Biol, Boulder, CO 80309 USA
关键词
D O I
10.1007/s00248-001-1057-x
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Previous work in an alpine dry meadow in the Front Range of the Rocky Mountains has shown that microbial biomass is high during winter and declines rapidly as snow melts in the spring, and that this decline is associated with changes in temperature regime and substrate availability. In this study we tested the hypothesis that the summer and winter microbial communities differ in function and composition. Shifts in species composition between pre- and post-snowmelt communities were detected using reciprocal hybridization of community DNA; DNA extracted from soils sampled at different times was significantly less homologous relative to spatial replicates sampled at the same time. Fungal/bacterial ratios, as measured by direct microscopic counts and by substrate-induced respiration experiments with specific inhibitors, were higher in winter soils. Specific activity of cellulase (absolute cellulase activity per unit microbial biomass C) was higher in the winter soils than in summer soils, while specific amylase activity was not different between winter and summer. Based on most-probable number measurements, the use of the phenolic compound vanillic acid was highest in the winter, while the use of the amino acid glycine was lowest in the winter. Winter and summer soil respiration responded differently to temperature; at 0degreesC, winter soils respired at a higher proportion of the 22degreesC rate than did summer soils.
引用
收藏
页码:307 / 314
页数:8
相关论文
共 36 条
[1]   ARGININE AMMONIFICATION, A SIMPLE METHOD TO ESTIMATE MICROBIAL ACTIVITY POTENTIALS IN SOILS [J].
ALEF, K ;
KLEINER, D .
SOIL BIOLOGY & BIOCHEMISTRY, 1986, 18 (02) :233-235
[2]  
[Anonymous], 1994, METHODS SOIL ANAL 2
[3]  
AUSEBEL FM, 1994, CURRENT PROTOCOLS MO
[4]   Seasonal changes in soil microbial communities along a fertility gradient of temperate grasslands [J].
Bardgett, RD ;
Lovell, RD ;
Hobbs, PJ ;
Jarvis, SC .
SOIL BIOLOGY & BIOCHEMISTRY, 1999, 31 (07) :1021-1030
[5]   COLORIMETRIC DETERMINATION OF OXIDIZABLE CARBON IN ACID SOIL SOLUTIONS [J].
BARTLETT, RJ ;
ROSS, DS .
SOIL SCIENCE SOCIETY OF AMERICA JOURNAL, 1988, 52 (04) :1191-1192
[6]   Determinants of soil microbial communities: Effects of agricultural management, season, and soil type on phospholipid fatty acid profiles [J].
Bossio, DA ;
Scow, KM ;
Gunapala, N ;
Graham, KJ .
MICROBIAL ECOLOGY, 1998, 36 (01) :1-12
[7]  
Brooks PD, 1996, BIOGEOCHEMISTRY, V32, P93, DOI 10.1007/BF00000354
[8]   Inorganic nitrogen and microbial biomass dynamics before and during spring snowmelt [J].
Brooks, PD ;
Williams, MW ;
Schmidt, SK .
BIOGEOCHEMISTRY, 1998, 43 (01) :1-15
[9]  
Brooks PD, 1997, OECOLOGIA, V110, P403, DOI [10.1007/PL00008814, 10.1007/s004420050175]
[10]  
BURNS SF, 1988, THESIS U COLORADO BO