Evaluation of capillary electrophoresis with post-column derivatization and laser-induced fluorescence detection for the determination of substance P and its metabolites

被引：61

作者：

Kostel, KL

Lunte, SM

机构：

[1] UNIV KANSAS,DEPT PHARMACEUT CHEM,LAWRENCE,KS 66047

[2] UNIV KANSAS,CTR BIOANALYT RES,LAWRENCE,KS 66047

来源：

JOURNAL OF CHROMATOGRAPHY B | 1997年 / 695卷 / 01期

关键词：

substance P; naphthalene-2,3-dicarboxaldehyde; beta-mercaptoethanol;

D O I：

10.1016/S0378-4347(97)00173-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method for the detection of substance P and its metabolites using capillary electrophoresis with post-capillary derivatization and laser-induced fluorescence detection is described. The post-capillary chemical derivatization system employs naphthalene-2,3-dicarboxaldehyde and beta-mercaptoethanol. Two reactor designs were evaluated for the determination of substance P and its metabolites. The fluorescent spectroscopic properties of the derivatives under optimal separation conditions were also examined. The final system was evaluated for the investigation of substance P metabolism in brain following perfusion of the striatum with substance P using microdialysis sampling.

引用

页码：27 / 38

页数：12

共 50 条

[1] FLUORESCENCE DETECTION IN CAPILLARY ELECTROPHORESIS - EVALUATION OF DERIVATIZING REAGENTS AND TECHNIQUES [J].