Measurement of salicylic acid in human serum using stable isotope dilution and gas chromatography-mass spectrometry

被引：19

作者：

Battezzati, A. ^{[1
]}

Fiorillo, G. ^{[1
]}

Spadafranca, A. ^{[1
]}

Bertoli, S. ^{[1
]}

Testolin, G. ^{[1
]}

机构：

[1] Univ Milan, Dipartimento Sci & Tecnol Alimentari & Microbiol, Int Ctr Assessment Nutr Status, I-20133 Milan, Italy

来源：

ANALYTICAL BIOCHEMISTRY | 2006年 / 354卷 / 02期

关键词：

salycilic acid; serum; stable isotopes; gas chromatography mass-spectrometry;

D O I：

10.1016/j.ab.2006.05.009

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A simple, highly selective, and sensitive method using stable isotope dilution and gas chromatography-mass spectrometry has been developed to quantify salicylic acid (SA) at concentrations naturally occurring in biological fluids, such as in the serum of subjects not taking aspirin. After extraction of liquid-liquid with diethyl ether and ethyl acetate and preparation of the tert-butyldimethylsilyl derivative, SA content was detected using deuterated SA as internal standard. The mean recovery of SA from serum was 85 +/- 6%. Intra- and interday precision and % relative error were < 15% in all cases. With a detection limit of 0.6 ng and a quantification limit of 2 ng, the method is therefore also adequate for population studies because of the small amount of blood necessary to perform the analyses. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：274 / 278

页数：5

共 17 条

[1]

Salicylic acid in the serum of subjects not taking aspirin. Comparison of salicylic acid concentrations in the serum of vegetarians, non-vegetarians, and patients taking low dose aspirin [J].