RNA replication by Q beta replicase: A working model

被引:61
作者
Brown, D [1 ]
Gold, L [1 ]
机构
[1] UNIV COLORADO,DEPT MOL CELLULAR & DEV BIOL,BOULDER,CO 80309
关键词
RNA-dependent RNA polymerase; viral replication;
D O I
10.1073/pnas.93.21.11558
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Two classes of RNA ligands that bound to separate, high affinity nucleic acid binding sites on Q beta replicase were previously identified. RNA ligands to the two sites, referred to as site I and site II, were used to investigate the molecular mechanism of RNA replication employed by the four-subunit replicase. Replication inhibition by site I- and site II-specific ligands defined two subsets of replicatable RNAs. When provided with appropriate 3' ends, ligands to either site served as replication templates. UV crosslinking experiments revealed that site I is associated with the S1 subunit, site II with elongation factor Tu, and polymerization with the viral subunit of the holoenzyme. These results provide the framework for a three site model describing template recognition and product strand initiation by Q beta replicase.
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页码:11558 / 11562
页数:5
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