Molecular Identification of Albumin and Hsp70 as Cytosolic Anandamide-Binding Proteins

被引：111

作者：

Oddi, Sergio ^{[1
,2
]}

Fezza, Filomena ^{[2
,3
]}

Pasquariello, Nicoletta ^{[1
]}

D'Agostino, Antonella ^{[1
]}

Catanzaro, Giuseppina ^{[1
,2
]}

De Simone, Chiara ^{[2
,3
]}

Rapino, Cinzia ^{[1
]}

Finazzi-Agro, Alessandro ^{[3
]}

Maccarrone, Mauro ^{[1
,2
]}

机构：

[1] Univ Teramo, Dept Biomed Sci, I-64100 Teramo, Italy

[2] Santa Lucia Fdn IRCCS, I-00143 Rome, Italy

[3] Univ Roma Tor Vergata, Dept Expt Med & Biochem Sci, I-00133 Rome, Italy

来源：

CHEMISTRY & BIOLOGY | 2009年 / 16卷 / 06期

关键词：

ACID AMIDE HYDROLASE; ENDOGENOUS CANNABINOID ANANDAMIDE; ENDOCANNABINOID SYSTEM; CELLULAR UPTAKE; SELECTIVE-INHIBITION; HUMAN KERATINOCYTES; AFFINITY BINDING; CB1; RECEPTOR; IN-VIVO; TRANSPORT;

D O I：

10.1016/j.chembiol.2009.05.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

The cellular uptake and the intracellular synthesis/degradation of anandamide are crucial steps for controlling its extracellular level and the duration of its activity. Although the biosynthesis and breakdown of anandamide are well understood, little is known about the mechanisms underlying its intracellular transport. Here, we investigated the presence of a potential carrier-mediated trafficking of anandamide within the cytosol, using a biotinylated analog as a tool to catch by affinity chromatography anandamide-interacting proteins. The identity of two of these anandamide-binding proteins, Hsp70 and serum albumin, was determined by mass spectrometry, confirmed by western blotting and confocal microscopy, and further validated through an anandamide-binding assay. These findings suggest that the trafficking of anandamide from the plasma membrane to the internal compartments of a cell occur via a non-vesicular mechanism mediated by cytosolic carriers.

引用

页码：624 / 632

页数：9

共 61 条

[1]

Functional role of high-affinity anandamide transport, as revealed by selective inhibition [J].