Adenoviral vector-mediated expression of physiologic levels of human factor VIII in nonhuman primates

被引：25

作者：

Brann, T ^{[1
]}

Kayda, D ^{[1
]}

Lyons, RM ^{[1
]}

Shirley, P ^{[1
]}

Roy, S ^{[1
]}

Kaleko, M ^{[1
]}

Smith, T ^{[1
]}

机构：

[1] Genet Therapy Inc, Gaithersburg, MD 20878 USA

来源：

HUMAN GENE THERAPY | 1999年 / 10卷 / 18期

关键词：

D O I：

10.1089/10430349950016401

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

An E1-, E2a-, E3-deleted adenoviral vector (Av3H82) encoding an epitope-tagged B domain-deleted human factor VIII cDNA (flagged FVIII) was evaluated in nonhuman primates. Twelve cynomolgus monkeys received intravenous administration of Av3H82; 6 monkeys received 6 x 10(11) particles/kg and another 6 received 3 x 10(12) particles/kg. Adenoviral vector transduction of the liver was efficient, reproducible, and linearly dose dependent. Physiologic levels of flagged:FVIII were readily detected in plasma samples obtained from monkeys that received the higher dose of vector and human FVIII mRNA was detected in their livers. Expression of transgene mRNA was restricted to the liver by, the albumin promoter. Although vector DNA was readily detected in the liver of monkeys that received the lower dose, neither human FVIII mRNA nor flagged FVIII protein could be detected, Vector distribution was widespread, with the highest levels observed in liver and spleen. Histopathology, hematology, and serum chemistry analysis identified the liver and blood a's major sites of toxicity, Transient mild serum elevations of liver enzymes were observed, along with a dose-dependent inflammatory response in the liver. In addition; mild lymphoid hyperplasia was observed in the spleen, Mild anemia and a transient decrease in platelet count were observed, as was marrow hyperplasia and extramedullary hematopoiesis.

引用

页码：2999 / 3011

页数：13

共 49 条

[1] TARGETED DISRUPTION OF THE MOUSE FACTOR-VIII GENE PRODUCES A MODEL OF HEMOPHILIA-A [J].