Acylpolyamines:: Mass spectrometric analytical methods for Araneidae spider acylpolyamines

被引：21

作者：

Itagaki, Y ^{[1
]}

Nakajima, T ^{[1
]}

机构：

[1] Suntory Inst Bioorgan Res, Osaka 618, Japan

来源：

JOURNAL OF TOXICOLOGY-TOXIN REVIEWS | 2000年 / 19卷 / 01期

关键词：

D O I：

10.1081/TXR-100100314

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

A new class of compounds, acylpolyamine has been isolated from spider venom constituents. Recent advances in highly sensitive mass spectrometric techniques have been applied successfully to characterize these acylpolyamines even with the use of a single venom gland. This has been achieved, in part, by improvements in fast atom bombardment mass spectrometry (FAB), continuous flow (FRIT) FAB-MS combined with reversed-phase high performance liquid chromatography (HPLC), matrix assisted laser desorption/ionization (MALDI) and high energy collision induced dissociation (CID) tandem MS/MS. Crude venom analysis without chromatographic separation can be realized directly by MALDI-MS. A charge-remote fragmentation method has provided abundant structure-related product ions and have reduced the quantity of required venom for the structure analysis of acylpolyamines. These mass spectrometric methods were proved to be useful for the analysis of complex constituents of spiders and other arthropod Venom glands.

引用

页码：23 / 52

页数：30

共 66 条

[1] CHARGE-REMOTE FRAGMENTATIONS - ANALYTICAL APPLICATIONS AND FUNDAMENTAL-STUDIES [J].