An RT-PCR test for the detection of tomato spotted wilt tospovirus incorporating immunocapture and colorimetric estimation

被引：19

作者：

Weekes, R ^{[1
]}

Barker, I ^{[1
]}

Wood, KR ^{[1
]}

机构：

[1] MAFF,CENT SCI LAB,YORK YO4 1LZ,N YORKSHIRE,ENGLAND

来源：

JOURNAL OF PHYTOPATHOLOGY-PHYTOPATHOLOGISCHE ZEITSCHRIFT | 1996年 / 144卷 / 11-12期

关键词：

D O I：

10.1111/j.1439-0434.1996.tb00301.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A reverse transcription-polymerase chain reaction (RT-PCR)-based assay for the detection of tomato spotted wilt virus (TSWV) has been modified and improved by the use of immunocapture of virus by solid phase adsorbed antibodies and colorimetric detection of nested PCR products, into which digoxigenin has been incorporated, by an ELOSA (enzyme-linked oligonucleotide-sorbent assay)-like assay. The use of the colorimetric assay for the detection of tospoviruses by using 'universal' primers in the RT-PCR stage is also described.

引用

收藏

页码：575 / 580

页数：6

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