Production of transgenic maize from bombarded type II callus: Effect of gold particle size and callus morphology on transformation efficiency

被引:124
作者
Frame, BR
Zhang, HY
Cocciolone, SM
Sidorenko, LV
Dietrich, CR
Pegg, SE
Zhen, SF
Schnable, PS
Wang, K [1 ]
机构
[1] Iowa State Univ, Dept Agron, Plant Transformat Facil, Ames, IA 50011 USA
[2] Iowa State Univ, Dept Zool & Genet, Ames, IA 50011 USA
关键词
zygotic embryo; regeneration; biolistic; Zea mays; Hi II callus; osmotic;
D O I
10.1007/s11627-000-0007-5
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Here we present a routine and efficient protocol for year-round production of fertile transgenic maize plants. Type II callus derived from maize Hi II immature zygotic embryos was transformed using the PDS 1000/He biolistic gun and selected on bialaphos. In an effort to improve the transformation protocol, the effects of gold particle size and callus morphology on transformation efficiency were investigated. Reducing gold particle size from 1.0 mu m or 0.6 mu m resulted in a significant increase in the rate of recovery of bialaphos-resistant clones from Type II callus. The average transformation efficiency of pre-embryogenic, early embryogenic and late embryogenic callus did not vary significantly. Rates of transformation, regeneration and fertility achieved for Type II callus are summarized and compared to those achieved for greenhouse- and field-derived immature zygotic embryos.
引用
收藏
页码:21 / 29
页数:9
相关论文
共 37 条
  • [1] Armstrong C. L., 1991, Maize Genetics Cooperation News Letter, P92
  • [2] Armstrong Charles L., 1994, P663
  • [3] FIELD-EVALUATION OF EUROPEAN CORN-BORER CONTROL IN PROGENY OF 173 TRANSGENIC CORN EVENTS EXPRESSING AN INSECTICIDAL PROTEIN FROM BACILLUS-THURINGIENSIS
    ARMSTRONG, CL
    PARKER, GB
    PERSHING, JC
    BROWN, SM
    SANDERS, PR
    DUNCAN, DR
    STONE, T
    DEAN, DA
    DEBOER, DL
    HART, J
    HOWE, AR
    MORRISH, FM
    PAJEAU, ME
    PETERSEN, WL
    REICH, BJ
    RODRIGUEZ, R
    SANTINO, CG
    SATE, SJ
    SCHULER, W
    SIMS, SR
    STEHLING, S
    TAROCHIONE, LJ
    FROMM, ME
    [J]. CROP SCIENCE, 1995, 35 (02) : 550 - 557
  • [4] ESTABLISHMENT AND MAINTENANCE OF FRIABLE, EMBRYOGENIC MAIZE CALLUS AND THE INVOLVEMENT OF L-PROLINE
    ARMSTRONG, CL
    GREEN, CE
    [J]. PLANTA, 1985, 164 (02) : 207 - 214
  • [5] Efficient transformation of scutellar tissue of immature maize embryos
    Brettschneider, R
    Becker, D
    Lorz, H
    [J]. THEORETICAL AND APPLIED GENETICS, 1997, 94 (6-7) : 737 - 748
  • [6] Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants
    Christensen, AH
    Quail, PH
    [J]. TRANSGENIC RESEARCH, 1996, 5 (03) : 213 - 218
  • [7] CHU CC, 1975, SCI SINICA, V18, P659
  • [8] COMPARISON OF SELECTIVE AGENTS FOR USE WITH THE SELECTABLE MARKER GENE BAR IN MAIZE TRANSFORMATION
    DENNEHEY, BK
    PETERSEN, WL
    FORDSANTINO, C
    PAJEAU, M
    ARMSTRONG, CL
    [J]. PLANT CELL TISSUE AND ORGAN CULTURE, 1994, 36 (01) : 1 - 7
  • [9] Dunder E., 1995, P127
  • [10] INHERITANCE AND EXPRESSION OF CHIMERIC GENES IN THE PROGENY OF TRANSGENIC MAIZE PLANTS
    FROMM, ME
    MORRISH, F
    ARMSTRONG, C
    WILLIAMS, R
    THOMAS, J
    KLEIN, TM
    [J]. BIO-TECHNOLOGY, 1990, 8 (09): : 833 - 839