Allele specific amplification of polymorphic sites for the detection of powdery mildew resistance loci in cereals

被引：22

作者：

Mohler, V ^{[1
]}

Jahoor, A ^{[1
]}

机构：

[1] TECH UNIV MUNICH, LEHRSTUHL PFLANZENBAU & PFLANZENZUCHTUNG, D-85350 Freising Weihenstephan, GERMANY

来源：

THEORETICAL AND APPLIED GENETICS | 1996年 / 93卷 / 07期

关键词：

allele-specific PCR (AS-PCR); powdery mildew; Hordeum vulgare; Triticum aestivum; sequence-tagged site (STS);

D O I：

暂无

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Primers for the polymerase chain reaction (PCR) were tailored to selectively amplify RFLP marker alleles associated with resistance and susceptibility for powdery mildew in cereals. The differentiation between marker alleles for susceptible and resistant genotypes is based on the discrimination of a single nucleotide by using allele-specific oligonucleotides as PCR primers. The PCR assays developed are diagnostic for RFLP alleles at the loci MWG097 in the barley genome and Whs350 in the wheat genome. The first marker locus is closely linked to MlLa resistance in barley, while the latter is linked to Pm2 resistance locus in wheat. PCR analysis of 31 barley and 30 wheat cultivars, with some exceptions, verified the presence or absence of the resistance loci investigated. These rapid PCR-based approaches are proposed as an efficient alternative to conventional procedures for selecting powdery mildew-resistant genotypes in breeding programs.

引用

页码：1078 / 1082

页数：5

共 27 条

[1] PCR ANALYSIS TO DISTINGUISH BETWEEN ALLELES OF A MEMBER OF A MULTIGENE FAMILY CORRELATED WITH WHEAT BREAD-MAKING QUALITY [J].