Improved method for non-denaturing polyacrylamide gradient gel electrophoresis for detection of small-sized LDL produced during postprandial hypertriglyceridaemia

被引：6

作者：

Ashida, Y

Takagi, A

Ikeda, Y

机构：

[1] Natl Cardiovasc Ctr, Res Inst, Dept Etiol & Pathophysiol, Osaka 5658565, Japan

[2] Natl Cardiovasc Ctr, Res Inst, Dept Pharmacol, Osaka 5658565, Japan

来源：

SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION | 1999年 / 59卷 / 08期

关键词：

alimentary; atherogenic; fat loading; fat ingestion; LDL size; lipoprotein; small dense LDL;

D O I：

10.1080/00365519950185166

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 [基础医学];

摘要：

A reliable method for detection of small-sized low-density lipoprotein (LDL) produced during transient hypertriglyceridaemia induced by ingestion of fat is described. Electrophoresis using a commercial non-denaturing 2.0-16% polyacrylamide gradient gel is commonly utilized to determine the size of LDL particles, but it failed to detect a minor change in LDL size induced during postprandial hypertriglyceridaemia. Detection of small-sized LDL was achieved by adjusting the polyacrylamide concentration. Electrophoresis using a nondenaturing 1.5-10% polyacrylamide gradient gel gave the best resolution for detecting small-sized LDL induced during postprandial hypertriglyceridaemia. This improved method may be useful for elucidating the underlying mechanism of production of small-sized LDL (small dense LDL) in subjects with chronic hypertriglyceridaemia.

引用

页码：663 / 669

页数：7

共 15 条

[1]

MODULATION OF LOW-DENSITY-LIPOPROTEIN SUBCLASSES BY ALIMENTARY LIPEMIA IN CONTROL AND NORMOTRIGLYCERIDEMIC NON-INSULIN-DEPENDENT DIABETIC SUBJECTS [J].