cDNA cloning of a developmentally regulated hemocyanin subunit in the crustacean Cancer magister and phylogenetic analysis of the hemocyanin gene family

被引:44
作者
Durstewitz, G
Terwilliger, NB
机构
[1] OREGON INST MARINE BIOL,CHARLESTON,OR 97420
[2] UNIV OREGON,DEPT BIOL,EUGENE,OR 97403
关键词
hemocyanin; parsimony analysis; phylogeny; Cancer magister; respiratory proteins; prophenoloxidases;
D O I
10.1093/oxfordjournals.molbev.a025762
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The complete cDNA sequence and protein reading frame of a developmentally regulated hemocyanin subunit in the Dungeness crab (Cancer magister) is presented. The protein sequence is aligned with 18 potentially homologous hemocyanin-type proteins displaying apparent sequence similarities. Functional domains are identified, and a comparison of predicted hydrophilicities, surface probabilities, and regional backbone flexibilities provides evidence for a remarkable degree of structural conservation among the proteins surveyed. Parsimony analysis of the protein sequence alignment identifies four monophyletic groups on the arthropodan branch of the hemocyanin gene tree: crustacean hemocyanins, insect hexamerins, chelicerate hemocyanins, and arthropodan prophenoloxidases. They form a monophyletic group relative to molluscan hemocyanins and nonarthropodan tyrosinases. Arthropodan prophenoloxidases, although functionally similar to tyrosinases, appear to belong to the arthropodan hexamer-type hemolymph proteins as opposed to molluscan hemocyanins and tyrosinases.
引用
收藏
页码:266 / 276
页数:11
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