Direct and electrically wired bioelectrocatalysis by hydrogenase from Thiocapsa roseopersicina

被引:39
作者
Morozov, SV
Karyakina, EE
Zorin, NA
Varfolomeyev, SD
Cosnier, S
Karyakin, AA
机构
[1] Moscow MV Lomonosov State Univ, Fac Chem, Moscow 119899, Russia
[2] Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142190, Russia
[3] CNRS, LEOPR, UMR 560, F-38041 Grenoble, France
关键词
hydrogenase; direct bioelectrocatalysis; enzyme electrodes; wired enzyme; polypyrrole;
D O I
10.1016/S1567-5394(01)00149-9
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Hydrogen enzyme electrodes based on direct and mediated bioelectrocatalysis were developed. Direct bioelectrocatalysis of hydrogen oxidation/evolution was observed for hydrogenase adsorbed on carbon filament material. The equilibrium hydrogen potential was achieved on mediatorless hydrogen enzyme electrodes in hydrogen atmosphere. The electrocatalytic activity of hydrogenase in direct bioelectrocatalysis of hydrogen oxidation was two orders of magnitude higher compared to platinum. The reported electrode remained 50% activity after 6 months of storage with periodical testing. Wired bioelectrocatalysis was achieved by adsorption of hydrogenase onto electropolymerized redox mediator N-methyl-N'-(12-pyrrol-1-yl-dodecyl)-4,4'-bipyridinium ditetrafluoroborate. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:169 / 171
页数:3
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