The biological effects of Auger electrons compared to alpha-particles and Li ions

被引:15
作者
Laster, BH
Shani, G
Kahl, SB
Warkentien, L
机构
[1] UNIV HOSP,DEPT RADIAT ONCOL,STONY BROOK,NY
[2] BEN GURION UNIV NEGEV,DEPT NUCL ENGN,IL-84105 BEER SHEVA,ISRAEL
[3] UNIV CALIF SAN FRANCISCO,DEPT PHARMACEUT CHEM,SAN FRANCISCO,CA 94143
关键词
D O I
10.3109/02841869609104046
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The present study reports the results of V-79 Chinese hamster cell survival studies in which Auger electron emission was stimulated in gadolinium (Gd) after thermal neutron capture, When a porphyrin that had previously been labeled with boron ((BOPP)-B-10) was also labeled with Gd (Gd-(BOPP)-B-10), the cells were incubated with Gd-(BOPP)-B-10 to assess the compound's ability to physiologically transport the Gd into the cell, and localize the Gd atoms in or near the cell's critical target, presumably the DNA. It was anticipated that Auger electron emission, stimulated during the Gd-157 (n, gamma) Gd-158 interaction, would impart additional high LET damage to that observed from the alpha-particle and Li ion during the B-10(n, alpha) Li-7 reaction, Following irradiation with thermal neutrons from the Brookhaven Medical Research Reactor, the effectiveness of the Auger electrons was determined by comparing the response of cells incubated with (BOPP)-B-10, where damage was imparted by the boron neutron capture (BNC) products, to that from Gd-(BOPP)-B-10, with equal concentration of B-10 in both solutions, An Auger effectiveness factor of similar to 2 was found for the Gd-(BOPP)-B-10 cells, The Auger effectiveness observed with Gd strongly suggested that the (BOPP)-B-10 molecule physiologically transported the Gd3+ ion intracellularly where it probably bound to DNA, Others have reported that Gd3+ does, in fact, complex with DNA, While depositing less energy per interaction than the high LET BNC reaction by-products, Auger electron ionization was more effective.
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收藏
页码:917 / 923
页数:7
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