Early postnatal alcohol exposure produced long-term deficits in brain weight, but not the number of neurons in the locus coeruleus

The locus coeruleus (LC), a small nucleus in the pontine tegmentum with clusters of norepinephrine (NE) containing neurons, projects to more brain regions than any other nucleus in the central nervous system. Therefore, any neuroanatomical deficits in the LC may have wide-ranging functional implications. Previous studies have shown that heavy alcohol exposure during development can damage several brain regions receiving extensive innervation from the LC (e.g., cerebellum and olfactory bulb). In this study, we examined the effects of early alcohol exposure during development on neuronal numbers in the LC of the adult rat. Sprague-Dawley rat pups were reared using artificial rearing techniques during the brain growth spurt period (part of the third trimester equivalent). The pups in the alcohol group received 4.5 g/kg per day in two of their 12 daily feedings from postnatal days (PDs) 4-9. Gastrostomy and suckle control groups were also included. On PD 90, the animals were sacrificed and their brains were processed for stereological cell counting. Whole brain weight, the number of neurons in the LC. the volume and neuronal density of the LC were assessed. There was a significant reduction in whole brain weight of the alcohol group compared with that of the gastrostomy control group, demonstrating a long lasting effect of alcohol on overall brain growth. Nevertheless, there were no differences in neuronal number, density and volume of the LC between alcohol and gastrostomy groups. Results were discussed in relation to the issues of temporal and regional vulnerabilities. (C) 1999 Elsevier Science B.V. All rights reserved.