Detection of Haemophilus influenzae and Streptococcus pneumoniae DNA in blood culture by a single PCR assay

被引：27

作者：

HassanKing, M

Baldeh, I

Adegbola, R

Omosigho, C

Usen, SO

Oparaugo, A

Greenwood, BM

机构：

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1996年 / 34卷 / 08期

关键词：

D O I：

10.1128/JCM.34.8.2030-2032.1996

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A multiplex PCR assay was developed to screen blood cultures from children in The Gambia with suspected pneumonia for the simultaneous detection of Haemophilus influenzae type b and Streptoccus pneumoniae isolates. Analysis of 295 blood cultures showed that PCR detected the organisms in all samples positive by culture in two samples infected with H. influenzae type b and four samples infected with S. pneumoniae that were culture negative, indicating that this method is sensitive for detecting these organisms in blood cultures.

引用

页码：2030 / 2032

页数：3

共 8 条

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