Ultradeep Pyrosequencing Detects Complex Patterns of CD8+ T-Lymphocyte Escape in Simian Immunodeficiency Virus-Infected Macaques

被引：56

作者：

Bimber, Benjamin N. ^{[1
]}

Burwitz, Benjamin J. ^{[2
]}

O'Connor, Shelby ^{[2
]}

Detmer, Ann ^{[1
]}

Gostick, Emma ^{[3
]}

Lank, Simon M. ^{[1
]}

Price, David A. ^{[3
]}

Hughes, Austin ^{[4
]}

O'Connor, David ^{[1
,2
]}

机构：

[1] Univ Wisconsin, Wisconsin Natl Primate Res Ctr, Madison, WI 53706 USA

[2] Univ Wisconsin, Dept Pathol & Lab Med, Madison, WI 53706 USA

[3] Cardiff Univ, Sch Med, Dept Med Biochem & Immunol, Cardiff, S Glam, Wales

[4] Univ S Carolina, Dept Biol Sci, Columbia, SC 29208 USA

来源：

JOURNAL OF VIROLOGY | 2009年 / 83卷 / 16期

基金：

英国医学研究理事会;

关键词：

ACUTE-PHASE; CLASS-I; DRUG-RESISTANCE; HIV-1; MUTATIONS; VIREMIA; CELLS; VARIANTS; VACCINE;

D O I：

10.1128/JVI.00897-09

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Human and simian immunodeficiency viruses (HIV/SIV) exhibit enormous sequence heterogeneity within each infected host. Here, we use ultradeep pyrosequencing to create a comprehensive picture of CD8(+) T-lymphocyte (CD8-TL) escape in SIV-infected macaques, revealing a previously undetected complex pattern of viral variants. This increased sensitivity enabled the detection of acute CD8-TL escape as early as 17 days postinfection, representing the earliest published example of CD8-TL escape in intrarectally infected macaques. These data demonstrate that pyrosequencing can be used to study the evolution of CD8-TL escape during immunodeficiency virus infection with an unprecedented degree of sensitivity.

引用

页码：8247 / 8253

页数：7

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