Regulation and immunohistochemical analysis of stress protein heme oxygenase-1 in rat kidney with myoglobinuric acute renal failure

被引：27

作者：

Ishizuka, S

Nagashima, Y

Numata, M

Yano, T

Hagiwara, K

Ozasa, H

Sone, M

Nihei, H

Horikawa, S

机构：

[1] TOKYO MED & DENT UNIV,MED RES INST,DEPT PATHOL BIOCHEM,CHIYODA KU,TOKYO 101,JAPAN

[2] TOKYO WOMENS MED COLL,DEPT INTERNAL MED 4,TOKYO 162,JAPAN

[3] YOKOHAMA CITY UNIV,SCH MED,DEPT PATHOL,YOKOHAMA,KANAGAWA 236,JAPAN

[4] NATL INST HLTH & NUTR,DIV APPL FOOD RES,TOKYO 162,JAPAN

[5] MINAMI IKEBUKURO CLIN,TOKYO 171,JAPAN

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 240卷 / 01期

关键词：

D O I：

10.1006/bbrc.1997.7573

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Intramuscular injection of hypertonic glycerol solution to rats results in acute renal injury. In this model, the proximal tubules are characteristically damaged. After glycerol injection renal glutathione (GSH) levels drastically decreased. On the other hand, stress protein heme oxygenase-1 (HO-1) was induced. When N-acetyl cysteine was administered to rats before 1 h glycerol injection, renal function was obviously improved, In this condition, the renal GSH content were sustained in the normal levels and HO-1 protein levels were decreased compared with those of glycerol-treated rats. Induction of HO-1 was accompanied by reduced renal GSH content. In addition, to investigate whether the location of HO-1 protein induced by glycerol injection is restricted to injured region or not in the kidney, we determined the localization of HO-1 protein using immunohistochemical staining, HO-1 protein was identified in the epithelia of the distal tubules, Henle's loop and collecting ducts, but not in the injured proximal tubules. (C) 1997 Academic Press.

引用

页码：93 / 98

页数：6

共 25 条

[1] INDUCTION OF HEME OXYGENASE IN TOXIC RENAL INJURY - A PROTECTIVE ROLE IN CISPLATIN NEPHROTOXICITY IN THE RAT [J].