Laminar optical tomography: demonstration of millimeter-scale depth-resolved imaging in turbid media

被引：108

作者：

Hillman, EMC ^{[1
]}

Boas, DA ^{[1
]}

Dale, AM ^{[1
]}

Dunn, AK ^{[1
]}

机构：

[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Martinos Ctr Biomed Imaging, Charlestown, MA 02129 USA

来源：

OPTICS LETTERS | 2004年 / 29卷 / 14期

关键词：

D O I：

10.1364/OL.29.001650

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

Laminar optical tomography (LOT) is a new technique that combines the advantages of diffuse optical tomography image reconstruction and a microscopy-based setup to allow noncontact imaging with 100-200-mum resolution effective over depths of 0-2.5 mm. LOT is being developed primarily for multispectral imaging of rat cortex, for which resolving functional dynamics in various layers of the brain's cortex (to depths of 1500 mum) is of increasing interest to neurophysiologists. System design and image reconstruction techniques are described, along with simulation and phantom results that demonstrate the characteristics and limitations of system accuracy and resolution. (C) 2004 Optical Society of America.

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页码：1650 / 1652

页数：3

相关论文

共 11 条

[1] Multiwavelength optical intrinsic signal imaging of cortical spreading depression [J].

Ba, AM ;

Guiou, M ;

Pouratian, N ;

Muthialu, A ;

Rex, DE ;

Cannestra, AF ;

Chen, JWY ;

Toga, AW .

JOURNAL OF NEUROPHYSIOLOGY, 2002, 88 (05) :2726-2735

[2]

BEVILACQUA F, 2002, OSA TRENDS OPTICS PH, V71, P677

[3] Intrinsic brain activity triggers trigeminal meningeal afferents in a migraine model [J].

Bolay, H ;

Reuter, U ;

Dunn, AK ;

Huang, ZH ;

Boas, DA ;

Moskowitz, MA .

NATURE MEDICINE, 2002, 8 (02) :136-142

[4] Transport-based image reconstruction in turbid media with small source-detector separations [J].

Dunn, A ;

Boas, D .

OPTICS LETTERS, 2000, 25 (24) :1777-1779

[5] Detecting AMD with Multiply Scattered Light Tomography [J].

A.E. Elsner ;

Q. Zhou ;

F. Beck ;

P.E. Tornambe ;

S.A. Burns ;

J.J. Weiter ;

A.W. Dreher .

International Ophthalmology, 2001, 23 (4-6) :245-250

[6] Laminar analysis of cerebral blood flow in cortex of rats by laser-Doppler flowmetry: A pilot study [J].

Fabricius, M ;

Akgoren, N ;

Dirnagl, U ;

Lauritzen, M .

JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 1997, 17 (12) :1326-1336

[7] On-line optical imaging of the human brain with 160-ms temporal resolution [J].

Franceschini, MA ;

Toronov, V ;

Filiaci, ME ;

Gratton, E ;

Fantini, S .

OPTICS EXPRESS, 2000, 6 (03) :49-57

[8] Concurrent optical imaging spectroscopy and laser-Doppler Flowmetry: The relationship between blood flow, oxygenation, and volume in rodent barrel cortex [J].

Jones, M ;

Berwick, J ;

Johnston, D ;

Mayhew, J .

NEUROIMAGE, 2001, 13 (06) :1002-1015

[9] Novel functional imaging technique from brain surface with optical coherence tomography enabling visualization of depth resolved functional structure in vivo [J].

Maheswari, RU ;

Takaoka, H ;

Kadono, H ;

Homma, R ;

Tanifuji, M .

JOURNAL OF NEUROSCIENCE METHODS, 2003, 124 (01) :83-92

[10] Spatiotemporal dynamics of sensory responses in layer 2/3 of rat barrel cortex measured in vivo by voltage-sensitive dye imaging combined with whole-cell voltage recordings and neuron reconstructions [J].

Petersen, CCH ;

Grinvald, A ;

Sakmann, B .

JOURNAL OF NEUROSCIENCE, 2003, 23 (04) :1298-1309