Molecular typing of Campylobacter strains using the cmp gene encoding the major outer membrane protein

被引:23
作者
Huang, Shouxiong
Luangtongkum, Taradon
Morishita, Teresa Y.
Zhang, Qijing
机构
[1] Ohio State Univ, Ohio Agr Res & Dev Ctr, Food Anim Hlth Res Program, Wooster, OH 44691 USA
[2] Ohio State Univ, Dept Vet Prevent Med, Columbus, OH 43210 USA
关键词
D O I
10.1089/fpd.2005.2.12
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
Thermophilic Campylobacter, particularly Campylobacter jejuni, is one of the major foodborne human pathogens of animal origin. Reliable and sensitive typing tools are required for understanding the epidemiology and ecology of this zoonotic bacteria agent. Currently, several molecular typing methods are available for differentiating Campylobacter strains, but each of them has limitations. Our previous study revealed that considerable sequence polymorphism exists in the cmp gene encoding the major outer membrane protein of Campylobacter and suggested that sequence variation of cmp may be utilized for discrimination of Campylobacter strains. In this study, we evaluated the feasibility of the cmp-based typing tool, using pulsed-field gel electrophoresis (PFGE) as the "gold" standard for comparison. The cmp alleles were sequenced from multiple Campylobacter strains, grouped, and compared with the PFGE profiles of these strains using Bionumerics. Results showed that 43 cmp sequence types and 43 PFGE types existed among the 60 Campylobacter isolates. Typeability of these strains is 100% using either the cmp-based method or PFGE. The discrimination indices are 0.973 for the cmp-based method and 0.969 for PFGE, respectively. The cmp sequence types are 77.6% congruent with the PFGE types. These results indicate that the cmp-based typing is a simple, yet highly discriminatory approach for molecular differentiation of C. jejuni strains.
引用
收藏
页码:12 / 23
页数:12
相关论文
共 34 条
[1]   Identification and characterisation of a cytotoxic porin-lipopolysaccharide complex from Campylobacter jejuni [J].
Bacon, DJ ;
Johnson, WM ;
Rodgers, FG .
JOURNAL OF MEDICAL MICROBIOLOGY, 1999, 48 (02) :139-148
[2]  
Bartie KL, 2000, J CLIN MICROBIOL, V38, P2141
[3]   CONFORMATIONAL-ANALYSIS OF THE CAMPYLOBACTER-JEJUNI PORIN [J].
BOLLA, JM ;
LORET, E ;
ZALEWSKI, M ;
PAGES, JM .
JOURNAL OF BACTERIOLOGY, 1995, 177 (15) :4266-4271
[4]   Evaluation of pulsed-field gel electrophoresis as a tool for determining the degree of genetic relatedness between strains of Escherichia coli O157:H7 [J].
Davis, MA ;
Hancock, DD ;
Besser, TE ;
Call, DR .
JOURNAL OF CLINICAL MICROBIOLOGY, 2003, 41 (05) :1843-1849
[5]   Computer-assisted analysis and epidemiological value of genotyping methods for Campylobacter jejuni and Campylobacter coli [J].
de Boer, P ;
Duim, B ;
Rigter, A ;
van der Plas, J ;
Jacobs-Reitsma, WF ;
Wagenaar, JA .
JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (05) :1940-1946
[6]   MOMP (major outer membrane protein) of Campylobacter jejuni;: a versatile pore-forming protein [J].
Dé, E ;
Jullien, M ;
Labesse, G ;
Pagès, JM ;
Molle, G ;
Bolla, JM .
FEBS LETTERS, 2000, 469 (01) :93-97
[7]   Genome sequence-based fluorescent amplified fragment length polymorphism of Campylobacter jejuni, its relationship to serotyping, and its implications for epidemiological analysis [J].
Desai, M ;
Logan, JMJ ;
Frost, JA ;
Stanley, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (11) :3823-3829
[8]   Multilocus sequence typing system for Campylobacter jejuni [J].
Dingle, KE ;
Colles, FM ;
Wareing, DRA ;
Ure, R ;
Fox, AJ ;
Bolton, FE ;
Bootsma, HJ ;
Willems, RJL ;
Urwin, R ;
Maiden, MCJ .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (01) :14-23
[9]   Determining confidence intervals when measuring genetic diversity and the discriminatory abilities of typing methods for microorganisms [J].
Grundmann, H ;
Hori, S ;
Tanner, G .
JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (11) :4190-4192
[10]   Evidence for recombination in the flagellin locus of Campylobacter jejuni: Implications for the flagellin gene typing scheme [J].
Harrington, CS ;
ThomsonCarter, FM ;
Carter, PE .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (09) :2386-2392