Secologanin synthase which catalyzes the oxidative cleavage of loganin into secologanin is a cytochrome P450

被引：86

作者：

Yamamoto, H

Katano, N

Ooi, A

Inoue, K

机构：

[1] Gifu Pharmaceut Univ, Dept Pharmacognosy, Gifu 5028585, Japan

[2] Nagasaki Univ, Sch Pharmaceut Sci, Lab Med Plants Garden, Nagasaki 8528521, Japan

来源：

PHYTOCHEMISTRY | 2000年 / 53卷 / 01期

关键词：

Lonicera japonica; Caprifoliaceae; cell suspension cultures; iridoid glucoside; secologanin; secologanin synthase; cytochrome P450; enzyme; biosynthesis;

D O I：

10.1016/S0031-9422(99)00471-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Secologanin synthase, an enzyme catalyzing the oxidative cleavage of the cyclopentane ring in loganin to form secologanin, was detected in microsomal preparations from cell suspension cultures of Lonicera japonica. The reaction required NADPH and molecular oxygen, and was blocked by carbon monoxide as well as by several other cytochrome P450 inhibitors, indicating that the reaction was mediated by cytochrome P450. Of the substrates examined, only specificity for loganin was demonstrated. A possible reaction mechanism is described. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：7 / 12

页数：6

共 23 条

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