Telomerase activity levels in the surgical margin and tumour distant tissue of the squamous cell carcinoma of the head-and-neck

被引:18
作者
Fabricius, EM
Gurr, U
Wildner, GP
机构
[1] Humboldt Univ, Fac Med, Clin Oral & Maxillofacial Surg, Charite, D-13353 Berlin, Germany
[2] Humboldt Univ, Fac Med, Charite, Robert Rossle Clin,Dept Pathol, D-13353 Berlin, Germany
关键词
head-and-neck cancer; squamous cell carcinoma; surgical tumour margin; telomerase; TRAP assay; ELISA;
D O I
10.1155/2002/452527
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The survival of patients with a head-and-neck squamous cell carcinoma is determined by loco-regional recurrence and second primary carcinomas. As a complement to histopathology, molecular changes of tumour marginal and tumour distant tissue may confirm curative surgical tumour extirpation. We tested telomerase activity with PCR-ELISA kits. 20 tumour margin biopsies were chosen by the surgeon from 20 patients. In addition, 3 tissue samples were taken from each of 20 additional patients, one from the carcinoma centre, the tumour margin and one distant from the tumour. 50% of the carcinoma centres were telomerase-positive. Thirteen of the 40 tumour margin samples showed increased telomerase levels, and in 3 of these residual carcinoma was histopathologically detected. Six of the 20 tumour distant tissues revealed increased telomerase levels. Telomerase positivity in carcinoma-free tumour margins correlated with a good prognosis. Confirmation of the results in a larger patient group is needed.
引用
收藏
页码:25 / 39
页数:15
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