Efflux systems and increased trypanothione levels in arsenite-resistant Leishmania

被引：83

作者：

Legare, D

Papadopoulou, B

Roy, G

Mukhopadhyay, R

Haimeur, A

Dey, S

Grondin, K

Brochu, C

Rosen, BP

Ouellette, M

机构：

[1] CHUL,SERV INFECTIOL,CTR RECH,CTR RECH INFECTIOL,ST FOY,PQ G1V 4G2,CANADA

[2] UNIV LAVAL,FAC MED,DEPT MICROBIOL,QUEBEC CITY,PQ G1K 7P4,CANADA

[3] WAYNE STATE UNIV,SCH MED,DEPT BIOCHEM & MOL BIOL,DETROIT,MI 48201

来源：

EXPERIMENTAL PARASITOLOGY | 1997年 / 87卷 / 03期

关键词：

ABC transporters; Leishmania; trypanothione; efflux systems; heavy metals;

D O I：

10.1006/expr.1997.4222

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The mechanism of resistance to the metal arsenite has been studied and compared in L. mexicana, L. tropica, and L. tarentolae selected in a step by step manner for arsenite resistance. Amplification of the ABC transporter gene pgpA was found to be a frequent resistance mechanism in all species. Transfection of pgpA genes into different species indicated that both the origin of the pgpA gene and the recipient strain into which the gene is transfected seem important for resistance. An increase in the levels of trypanothione was also correlated with metal resistance in different Leishmania species. The mechanism used to increase the levels of trypanothione seems to differ, however, between the different species. This study points to a key role of transporters and thiol levels in metal resistance in Leishmania. (C) 1997 Academic Press.

引用

页码：275 / 282

页数：8

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