Imaging of dynamic alteration of functional structures

被引：1

作者：

Singer, M ^{[1
]}

Kessler, M ^{[1
]}

Boehnert, M ^{[1
]}

机构：

[1] Univ Erlangen Nurnberg, Inst Physiol & Cardiol, Erlangen, Germany

来源：

FUNCTIONAL MONITORING AND DRUG-TISSUE INTERACTION | 2002年 / 4623卷

关键词：

Monitoring of alterations of subcellular structures in tissue by use of optical sensors;

D O I：

10.1117/12.469462

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 [生物医学工程];

摘要：

Subcellular structures of liver are mainly caused by organelles of hepatocytes and endothelial cells when VIS and NIR are used for irradiation. Total tissue anoxia induces very fast shrinking of mitochondria by switching the oxygenator of the perfusion system from normoxia to total anoxia. This oxygen reaction causes a drastic decrease of backscattered light. For further investigations we used a protocol of three different conditions: 1. Stimulation of subcellular structures in Hb-free perfused liver by application of hypoxia and normoxia, 2. Effects of erythrocytes, 3. Measurements of tissue pigments. Investigations performed by use of our 2D- and 3D-imaging system revealed that the subcellular space with its organelles represents a highly dynamic system.

引用

页码：308 / 313

页数：6