Investigations on the Leaf Surface Ultrastructure in Grapevine (Vitis vinifera L.) by Scanning Microscopy

被引：10

作者：

Bensalem-Fnayou, A. ^{[1
]}

Jellouli, N. ^{[1
]}

Bouamama, B. ^{[1
]}

Mliki, A. ^{[1
]}

Ghorbel, A. ^{[1
]}

机构：

[1] Ctr Biotechnol Borj Cedria, Lab Physiol Mol Vigne, Hammam Lif 2050, Tunisia

来源：

SCANNING | 2009年 / 31卷 / 03期

关键词：

SEM; ESEM; AFM; grapevine; leaf surface; epicuticular wax; ATOMIC-FORCE MICROSCOPY; ELECTRON-MICROSCOPY; EPICUTICULAR WAXES; ENVIRONMENTAL SEM; MORPHOLOGY; LEAVES;

D O I：

10.1002/sca.20145

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

Several Scanning microscopy techniques were used to investigate the leaf surface ultrastructure in the local "Razegui" grapevine cultivar (Vitis vinifera L.). Conventional scanning electron microscopy performed oil glutaraldehyde-fixed samples allowed observation of well-preserved epidermal cells with an overlaying waxy layer. At a high magnification, the waxy layer exhibited crystalline projections in the form of horizontal and vertical platelets. Also, to avoid eventual ultrastructural alterations inherent in the use of solvents during sample preparation, fresh leaf blade samples were directly observed by environmental scanning electron microscopy. A classical image of convex living epidermal cells was observed. At 2400 x magnification, epicuticular waxes exhibited a granular structure. However, high-magnification images were not obtained with this device. The atomic force microscopy (AFM) performed on fresh leaf blade samples allowed observation of a textured surface and heterogeneous profiles attributed to epicuticular wax deposits. AFM topography images confirmed further, the presence of irregular crystalloid wax projections as multishaped platelets on the adaxial surface of grapevine leaf. SCANNING 31: 127-131,2009. (C) 2009 Wiley Periodicals, Inc.

引用

页码：127 / 131

页数：5

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