ALEX® (artificial liver for extracorporeal xenoassistance):: A new bioreactor containing a porcine autologous biomatrix as hepatocyte support.: Preliminary results in an ex vivo experimental model

被引:6
作者
Ambrosino, G
Varotto, S
Basso, S
Galavotti, D
Cecchetto, A
Carraro, P
Naso, A
De Silvestro, G
Plebani, M
Giron, G
Abatangelo, G
Donato, D
Braga, GP
Cestrone, A
Marrelli, L
Trombetta, M
Lorenzelli, V
Picardi, A
Valente, ML
Palu, G
Colantoni, A
Van Thiel, D
Ricordi, C
D'Amico, DF
机构
[1] Univ Padua, Sect Gen & Liver Transplant Surg, Dept Surg & Gastroenterol Sci, Liver Transplantat Unit,Sch Med, I-35128 Padua, Italy
[2] Univ Padua, Dept Pathol, I-35128 Padua, Italy
[3] Univ Padua, Unit Lab & Chem Anal, I-35128 Padua, Italy
[4] Univ Padua, Dept Nephrol, I-35128 Padua, Italy
[5] Univ Padua, Transfus Unit, I-35128 Padua, Italy
[6] Univ Padua, Dept Anesthesiol & Pharmacol, I-35128 Padua, Italy
[7] Univ Padua, Dept Histol & Tissue Engn, I-35128 Padua, Italy
[8] Univ Miami, Sch Med, Diabet Res Inst, Coral Gables, FL 33124 USA
[9] RanD Srl, Cavezzo, MO, Italy
[10] Univ Hosp Padova, Med Off, Azienda Osped, Padua, Italy
[11] Gen Hosp, Med Off, Padua, Italy
[12] Univ Roma La Sapienza, Dept Chem Engn, I-00185 Rome, Italy
[13] Univ Campus Bio Med, Dept Biomat Engn, Rome, Italy
[14] Univ Campus Bio Med, Dept Internal Med, Rome, Italy
[15] Loyola Univ, Dept Gastroenterol & Liver Transplant, Chicago, IL 60611 USA
[16] Univ Padua, Dept Pathol, I-35128 Padua, Italy
[17] Univ Padua, Dept Microbiol, I-35128 Padua, Italy
关键词
bioartificial liver; biomatrix; bioreactor; liver;
D O I
10.1177/039139880202501010
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Long-term maintenance of viability and expression of differentiated hepatocyte function is crucial for bioartificial liver support. We developed a new bioreactor design (ALEX(R)), associated with a new extracellular autologous hepatocyte biomatrix (Porcine Autologous Biomatrix - PBM) support. To test this new bioreactor, we compared it to a standard BAL (Bio-Artificial Liver) cartridge in a ex vivo model using human plasma added to bilirubin, ammonium and lidocaine. A pathology study was performed on both bioreactors. The results suggest that ALEX(R) allows a maximal contact between the perfusing plasma and the liver cells and a proper hepatocyte support by a cell-to-matrix attachment. ALEX(R) is a suitable cell support bioreactor, guaranteeing long-term maintenance of the metabolic activity of hepatocytes when compared to a standard BAL cartridge.
引用
收藏
页码:960 / 965
页数:6
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