Oct4, a Novel Marker for Human Gastric Cancer

Background and Objective: Octamer-4 (Oct4), a transcription factor involved in regulating human embryonic stern cells (ESCs), may play a role in tumorigenesis. Since little is known about the efficacy of Oct4 its a potential biomarker for gastric cancer (GC), we investigated its expression in GC tissues and its relationship to various clinicopathological parameters. Methods: Primary tumor tissues and matching, adjacent non-cancerous tissues were obtained from 62 GC patients, and Oct4 expression was examined by reverse transcription-PCR (RT-PCR) and real-time PCR. Twenty biopsy specimens of atrophic gastritis and gastric ulcer individually were collected as control. To detect Oct4 expression in the paired GC and non-cancerous tissues at the protein level, Western blotting and immunohistochemistry (IHC) were employed. Correlation analyses were conducted to assess the relationship between Oct4 expression and clinicopathological parameters. Results: Oct4 expression levels were higher in GC tissues compared to matching, adjacent non-cancerous tissues, atrophic gastritis and gastric ulcer tissues. Additionally. Oct4 expression in GC tumors correlated with their differentiation status. but not with patient age or gender, tumor size, TNM stage. depth of invasion, Or the presence of lymph node metastasis. Conclusions: Oct4 may be a potential biomarker for the initiation, progression. and differentiation of human GC. J. Surg. Oncol. 2009;99:414-419. (C) 2009 Wiley-Liss, Inc.