Phytochelatin homologs induced in hairy roots of horseradish

被引：47

作者：

Kubota, H ^{[1
]}

Sato, K ^{[1
]}

Yamada, T ^{[1
]}

Maitani, T ^{[1
]}

机构：

[1] Natl Inst Hlth Sci, Tokyo 1588501, Japan

来源：

PHYTOCHEMISTRY | 2000年 / 53卷 / 02期

关键词：

Armoracia rusticana; cadmium; cell culture; cruciferae; horseradish; metallothionein; phytochelatin;

D O I：

10.1016/S0031-9422(99)00493-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When exposed to excess heavy metals, plants induce phytochelatins and related peptides (all designated as PCAs). Thus, when hairy roots of horseradish (Armoracia rusticana) were exposed for 3 days to cadmium (1 mM) along with reduced glutathione (2 mM), PCA induction occurred. Moreover, a new family of thiol peptides was detected as well as the previously known PCAs, as revealed by postcolumn-derivatization HPLC. Two were isolated and their structures were identified as (gamma-Glu-Cys)(n)-Gln (n = 3 and 4) by electrospray ionization-mass spectrometer spectra, this being confirmed by chemical synthesis of the peptides. These new analogs constitute the sixth PCA family identified to date. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：239 / 245

页数：7

共 22 条

[1]

Binz PA, 1999, METALLOTHIONEIN IV, P7

[2]

GEKELER W, 1989, Z NATURFORSCH C, V44, P361

[3] HOMO-PHYTOCHELATINS ARE HEAVY METAL-BINDING PEPTIDES OF HOMO-GLUTATHIONE CONTAINING FABALES [J].