Sequential observation of liver cell regeneration after massive hepatic necrosis in auxiliary partial orthotopic liver transplantation

被引:47
作者
Fujita, M
Furukawa, H
Hattori, M
Todo, S
Ishida, Y
Nagashima, K
机构
[1] Hokkaido Univ, Sch Med, Lab Mol & Cellular Pathol, Kita Ku, Sapporo, Hokkaido 0608638, Japan
[2] Hokkaido Univ Hosp, Dept Surg Pathol, Sapporo, Hokkaido, Japan
[3] Hokkaido Univ Hosp, Dept Surg, Sapporo, Hokkaido, Japan
[4] Japan Sci & Technol Corp, Core Res Evolut Sci & Technol, Tokyo, Japan
关键词
auxiliary partial orthotopic liver transplantation; ductular hepatocytes; fulminant hepatitis; liver cell regeneration;
D O I
10.1038/modpathol.3880029
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
The morphogenesis of hepatocytes after massive hepatic necrosis to recovery through Liver cell regeneration has not been fully understood. Sequential biopsies were performed on the native liver of a 22-year-old man who underwent auxiliary partial orthotopic liver transplantation 1 month after fulminant hepatitis. Auxiliary partial orthotopic liver transplantation was successful, and the biopsy samples permitted us to examine the regenerating process of hepatocytes after massive necrosis, At the time of auxiliary partial orthotopic liver transplantation (postoperative day 0), 95% of hepatocytes were lost and a few ductules were found in the portal areas. The ductules stained with cytokeratin 19. At postoperative day 7, the ductules began to increase in size and number and became dilated over a period of 1 month, when individual hepatocytes with clear cytoplasm appeared from the ductules, As the differentiation of hepatocytes increased, the expression of cytokeratin 19 was found to decrease. From 2 to 3 months, all of the ductules were transformed into hepatocytes, and they began to form round cell clusters. From 3 to 6 months, the round cell clusters became organized into trabecula with fibrosis. From 6 to 12 months, a lobular architecture was established, and by 14 months, the necrotic liver was fully recovered to normal. This study by examination of sequential biopsies demonstrates the progression of the regenerating process from total hepatic necrosis to complete recovery.
引用
收藏
页码:152 / 157
页数:6
相关论文
共 15 条
  • [1] Auxiliary partial orthotopic liver transplantation for fulminant hepatitis - The Paul Brousse Experience
    Bismuth, H
    Azoulay, D
    Samuel, D
    Reynes, M
    Grimon, G
    Majno, P
    Castaing, D
    [J]. ANNALS OF SURGERY, 1996, 224 (06) : 712 - 726
  • [2] AUXILIARY LIVER-TRANSPLANTATION FOR FULMINANT AND SUBFULMINANT HEPATIC-FAILURE
    BOUDJEMA, K
    CHERQUI, D
    JAECK, D
    CHENARDNEU, MP
    STEIB, A
    FREIS, G
    BECMEUR, F
    BRUNOT, B
    SIMEONI, U
    BELLOCQ, JP
    TEMPE, JD
    WOLF, P
    CINQUALBRE, J
    [J]. TRANSPLANTATION, 1995, 59 (02) : 218 - 223
  • [3] ChenardNeu MP, 1996, HEPATOLOGY, V23, P1119
  • [4] Demetris AJ, 1996, AM J PATHOL, V149, P439
  • [5] Fiel MI, 1997, MODERN PATHOL, V10, P348
  • [6] AUXILIARY PARTIAL ORTHOTOPIC LIVER-TRANSPLANTATION (APOLT) FOR FULMINANT HEPATIC-FAILURE - 1ST SUCCESSFUL CASE-REPORT
    GUBERNATIS, G
    PICHLMAYR, R
    KEMNITZ, J
    GRATZ, K
    [J]. WORLD JOURNAL OF SURGERY, 1991, 15 (05) : 660 - 666
  • [7] Haque S, 1996, LAB INVEST, V75, P699
  • [8] LEE RG, 1994, DIAGNOSTIC LIVER PAT, P23
  • [9] RECOVERY OF FAILING LIVER AFTER AUXILIARY HETEROTOPIC TRANSPLANTATION
    METSELAAR, HJ
    HESSELINK, EJ
    DERAVE, S
    TENKATE, FJW
    LAMERIS, JS
    GROENLAND, THN
    REUVERS, CB
    WEIMAR, W
    TERPSTRA, OT
    SCHALM, SW
    [J]. LANCET, 1990, 335 (8698) : 1156 - 1157
  • [10] HETEROTOPIC LIVER-TRANSPLANTATION FOR FULMINANT HEPATIC-FAILURE - A BRIDGE TO RECOVERY
    MORITZ, MJ
    JARRELL, BE
    ARMENTI, V
    RADOMSKI, J
    CARABASI, RA
    ZEITOUN, G
    COLUMBUS, K
    RUBIN, R
    MUNOZ, S
    MADDREY, W
    [J]. TRANSPLANTATION, 1990, 50 (03) : 524 - 526