Identification of porin as a binding site for MAP2

被引：80

作者：

Linden, M

Karlsson, G

机构：

[1] Department of Biochemistry, University of Stockholm

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1996年 / 218卷 / 03期

关键词：

D O I：

10.1006/bbrc.1996.0148

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Porin or VDAC, the pore forming protein in the outer mitochondrial membrane, was identified as a binding site for microtubule-associated protein 2 (MAP2). By affinity chromatography using covalently bound MAP2, two proteins were selectively purified with molecular weights 63 and 70 KD. The 63 KD protein was identified to be the dimer of porin using affinity purified porin antibodies. (C) 1996 Academic Press, Inc.

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页码：833 / 836

页数：4

相关论文

共 18 条

[1] STUDIES ON HUMAN PORIN .6. PRODUCTION AND CHARACTERIZATION OF 8 MONOCLONAL MOUSE ANTIBODIES AGAINST THE HUMAN VDAC PORIN 31HL AND THEIR APPLICATION FOR HISTOTOPOLOGICAL STUDIES IN HUMAN SKELETAL-MUSCLE [J].

BABEL, D ;

WALTER, G ;

GOTZ, H ;

THINNES, FP ;

JURGENS, L ;

KONIG, U ;

HILSCHMANN, N .

BIOLOGICAL CHEMISTRY HOPPE-SEYLER, 1991, 372 (12) :1027-1034

[2] SUBFRACTIONATION OF THE OUTER-MEMBRANE OF RAT-BRAIN MITOCHONDRIA - EVIDENCE FOR THE EXISTENCE OF A DOMAIN CONTAINING THE PORIN-HEXOKINASE COMPLEX [J].

DORBANI, L ;

JANCSIK, V ;

LINDEN, M ;

LETERRIER, JF ;

NELSON, BD ;

RENDON, A .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1987, 252 (01) :188-196

[3]

Forman DS, 1987, Axonal Transport, P155

[4] CROSS-LINKER SYSTEM BETWEEN NEUROFILAMENTS, MICROTUBULES, AND MEMBRANOUS ORGANELLES IN FROG AXONS REVEALED BY THE QUICK-FREEZE, DEEP-ETCHING METHOD [J].

HIROKAWA, N .

JOURNAL OF CELL BIOLOGY, 1982, 94 (01) :129-142

[5] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[6] INTERACTIONS BETWEEN BRAIN MITOCHONDRIA AND CYTOSKELETON - EVIDENCE FOR SPECIALIZED OUTER-MEMBRANE DOMAINS INVOLVED IN THE ASSOCIATION OF CYTOSKELETON-ASSOCIATED PROTEINS TO MITOCHONDRIA IN-SITU AND IN-VITRO [J].

LETERRIER, JF ;

RUSAKOV, DA ;

NELSON, BD ;

LINDEN, M .

MICROSCOPY RESEARCH AND TECHNIQUE, 1994, 27 (03) :233-261

[7] AGE-DEPENDENT CHANGES IN THE ULTRASTRUCTURE AND IN THE MOLECULAR COMPOSITION OF RAT-BRAIN MICROTUBULES [J].

LETERRIER, JF ;

EYER, J .

JOURNAL OF NEUROCHEMISTRY, 1992, 59 (03) :1126-1137

[8] STUDIES ON THE INTERACTION BETWEEN MITOCHONDRIA AND THE CYTOSKELETON [J].

LINDEN, M ;

NELSON, BD ;

LONCAR, D ;

LETERRIER, JF .

JOURNAL OF BIOENERGETICS AND BIOMEMBRANES, 1989, 21 (04) :507-518

[9] PURIFICATION OF A PROTEIN HAVING PORE FORMING ACTIVITY FROM THE RAT-LIVER MITOCHONDRIAL OUTER-MEMBRANE [J].

LINDEN, M ;

GELLERFORS, P ;

NELSON, BD .

BIOCHEMICAL JOURNAL, 1982, 208 (01) :77-82

[10] THE SPECIFIC BINDING OF THE MICROTUBULE-ASSOCIATED PROTEIN-2 (MAP2) TO THE OUTER-MEMBRANE OF RAT-BRAIN MITOCHONDRIA [J].

LINDEN, M ;

NELSON, BD ;

LETERRIER, JF .

BIOCHEMICAL JOURNAL, 1989, 261 (01) :167-173