SNP genotyping by using photochemical ligation

被引：50

作者：

Ogasawara, Shinzi

Fujimoto, Kenzo ^{[1
]}

机构：

[1] Japan Adv Inst Sci & Technol, Sch Mat Sci, Ishikawa 9231292, Japan

[2] Japan Sci & Technol Agcy, PRESTO, Kawaguchi 3320012, Japan

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2006年 / 45卷 / 27期

关键词：

biosensors; DNA recognition; ligation reactions; medicinal chemistry; photochemistry;

D O I：

10.1002/anie.200600790

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) A chip for single mismatches: Results obtained with a photochemical-ligation-based DNA chip for enhanced discrimination of single-nucleotide polymorphisms (see scheme) show that the reaction takes place over a period of hours, with the 50% stage reached within 10 minutes. A single mismatch in the target sequence yielded extremely little ligated product (a rate 103-fold lower than for the complementary case). © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.

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页码：4512 / 4515

页数：4

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