Mobility of synaptic vesicles in different pools in resting and stimulated frog motor nerve terminals

被引:79
作者
Gaffield, Michael A.
Rizzoli, Silvio O.
Betz, William J. [1 ]
机构
[1] Univ Colorado, Sch Med, Dept Physiol & Biophys, Aurora, CO 80045 USA
[2] Univ Colorado, Neurosci Program, Sch Med, Aurora, CO 80045 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1016/j.neuron.2006.06.031
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We used fluorescence recovery after photobleaching (FRAP) to measure the mobility of synaptic vesicles in frog motor nerve terminals. Vesicles belonging to the recycling pool or to the reserve pool were selectively labeled with FM1-43. In resting terminals, vesicles in the reserve pool were immobile, while vesicles in the recycling pool were mobile. Nerve stimulation increased the mobility of reserve pool vesicles. Treatment with latrunculin A, which destroyed actin filaments, had no significant effect on mobility, and reducing the temperature likewise had little effect, suggesting that recycling pool vesicles move by simple diffusion. Application of okadaic acid caused vesicle mobility in both pools to increase to the same level. We could model these and others' results quantitatively by taking into account the relative numbers of mobile and immobile vesicles in each pool, and vesicle packing density, which has a large effect on mobility.
引用
收藏
页码:317 / 325
页数:9
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