Real-time tissue characterization on the basis of in vivo Raman spectra

被引:74
作者
Schut, TCB [1 ]
Wolthuis, R [1 ]
Caspers, PJ [1 ]
Puppels, GJ [1 ]
机构
[1] Univ Hosp Rotterdam Dijkzigt, Erasmus Med Ctr, Dept Gen Surg, Lab Intens Care Res & Opt Spect, NL-3015 GD Rotterdam, Netherlands
关键词
D O I
10.1002/jrs.852
中图分类号
O433 [光谱学];
学科分类号
0703 ; 070302 ;
摘要
The application of in vivo Raman spectroscopy for clinical diagnosis demands dedicated software that can perform the necessary signal processing and subsequent (multivariate) data analysis, enabling clinically relevant parameters to be extracted and made available in real time. Here we describe the design and implementation of a software package that allows for real-time signal processing and data analysis of Raman spectra. The design is based on automatic data exchange between Grams, a spectroscopic data acquisition and analysis program, and Matlab, a program designed for array-based calculations. The data analysis software has a modular design providing great flexibility in developing custom data analysis routines for different applications. The implementation is illustrated by a computationally demanding application for the classification of skin spectra using principal component analysis and linear discriminant analysis. Copyright (C) 2002 John Wiley Sons, Ltd.
引用
收藏
页码:580 / 585
页数:6
相关论文
共 13 条
[1]   In vivo determination of the molecular composition of artery wall by intravascular Raman spectroscopy [J].
Buschman, HP ;
Marple, ET ;
Wach, ML ;
Bennett, B ;
Schut, TCB ;
Bruining, HA ;
Bruschke, AV ;
van der Laarse, A ;
Puppels, GJ .
ANALYTICAL CHEMISTRY, 2000, 72 (16) :3771-3775
[2]   Investigation of skin and skin lesions by NIR-FT-Raman spectroscopy [J].
Fendel, S ;
Schrader, B .
FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 1998, 360 (05) :609-613
[3]   Structure of water, proteins, and lipids in intact human skin, hair, and nail [J].
Gniadecka, M ;
Nielsen, OF ;
Christensen, DH ;
Wulf, HC .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1998, 110 (04) :393-398
[4]   Prospects for in vivo Raman spectroscopy [J].
Hanlon, EB ;
Manoharan, R ;
Koo, TW ;
Shafer, KE ;
Motz, JT ;
Fitzmaurice, M ;
Kramer, JR ;
Itzkan, I ;
Dasari, RR ;
Feld, MS .
PHYSICS IN MEDICINE AND BIOLOGY, 2000, 45 (02) :R1-R59
[5]  
Lawson EE, 1997, J RAMAN SPECTROSC, V28, P111, DOI 10.1002/(SICI)1097-4555(199702)28:2/3<111::AID-JRS87>3.0.CO
[6]  
2-Z
[7]  
PUPPELS GJ, 1998, P SOC PHOTO-OPT INS, V3257, P787
[8]  
PUPPELS GJ, 2001, HDB RAMAN SPECTROSCO, P549
[9]   In vivo detection of dysplastic tissue by Raman spectroscopy [J].
Schut, TCB ;
Witjes, MJH ;
Sterenborg, HJCM ;
Speelman, OC ;
Roodenburg, JLN ;
Marple, ET ;
Bruining, HA ;
Puppels, GJ .
ANALYTICAL CHEMISTRY, 2000, 72 (24) :6010-6018
[10]  
Shim MG, 2000, PHOTOCHEM PHOTOBIOL, V72, P146, DOI 10.1562/0031-8655(2000)072<0146:IVNIRS>2.0.CO