Some Cyclin-Dependent Kinase Inhibitors-Related Genes Are Regulated by Vitamin C in a Model of Diet-Induced Obesity

被引：17

作者：

Boque, Noemi ^{[1
]}

Campion, Javier ^{[1
]}

Ignacio Milagro, Fermin ^{[1
]}

Moreno-Aliaga, Maria-Jesus ^{[1
]}

Alfredo Martinez, Jose ^{[1
]}

机构：

[1] Univ Navarra, Inst Nutr & Food Sci, Pamplona 31008, Spain

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2009年 / 32卷 / 08期

关键词：

cyclin-dependent kinase inhibitor; cafeteria diet; ascorbic acid; adipose tissue; fatty liver; CELL-CYCLE; OXIDATIVE STRESS; DOWN-REGULATION; ADIPOSE-TISSUE; EXPRESSION; DEGRADATION; ACTIVATION; P57(KIP2); P53; P27(KIP1);

D O I：

10.1248/bpb.32.1462

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The aim of this research was to investigate differential gene expression of cyclin-dependent kinase inhibitors (CKIs) in white adipose tissue (WAT) and liver from high-fat fed male Wistar rats with or without vitamin C (VC) supplementation (750 mg/kg of body weight). After 56 d of experimentation, animals fed on a cafeteria diet increased significantly body weights and total body fat. Reverse transcription-polymerase chain reaction (RTPCR) studies showed that cafeteria diet decreased p21 and p57 mRNA expression in subcutaneous WAT and increased p21 mRNA in liver. Overall, these data provide new information about the role of high fat intake on mRNA levels of several CKIs with implications in adipogenesis, cell metabolism and weight homeostasis. Interestingly, VC supplementation partially prevented diet-induced adiposity and increased p27 mRNA in liver without any changes in the other tissues and genes analyzed. Thus, hepatic mRNA changes induced by ascorbic acid indicate a possible role of these genes in diet-induced oxidative stress processes.

引用

页码：1462 / 1468

页数：7

共 54 条

[1] Post-translational modifications and activation of p53 by genotoxic stresses [J].

Appella, E ;

Anderson, CW .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2001, 268 (10) :2764-2772

[2] Evidence for cytosolic p27(Kip1) ubliquitylation and degradation during adipocyte hyperplasia [J].

Auld, Corinth A. ;

Morrison, Ron F. .

OBESITY, 2006, 14 (12) :2136-2144

[3] Cell cycle regulation by the retinoblastoma family of growth inhibitory proteins [J].

Beijersbergen, RL ;

Bernards, R .

BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER, 1996, 1287 (2-3) :103-120

[4] Free fatty acids are involved in the inverse relationship between hormone-sensitive lipase (HSL) activity and expression in adipose tissue after high-fat feeding or β3-adrenergic stimulation [J].

Berraondo, B ;

Martínez, JA .

OBESITY RESEARCH, 2000, 8 (03) :255-261

[5] Role of the SCFSkp2 ubiquitin ligase in the degradation of p21Cip1 in S phase [J].

Bornstein, G ;

Bloom, J ;

Sitry-Shevah, D ;

Nakayama, K ;

Pagano, M ;

Hershko, A .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (28) :25752-25757

[6]

Campión J, 2006, J PHYSIOL BIOCHEM, V62, P71, DOI 10.1007/BF03174068

[7] Vitamin C supplementation influences body fat mass and steroidogenesis-related genes when fed a high-fat diet [J].

Campion, Javier ;

Milagro, Fermin I. ;

Fernandez, Dietmar ;

Martinez, J. Alfredo .

INTERNATIONAL JOURNAL FOR VITAMIN AND NUTRITION RESEARCH, 2008, 78 (02) :87-95

[8] Interaction of p21CDKN1A with PCNA regulates the histone acetyltransferase activity of p300 in nucleotide excision repair [J].

Cazzalini, Ornella ;

Perucca, Paola ;

Savio, Monica ;

Necchi, Daniela ;

Bianchi, Livia ;

Stivala, Lucia A. ;

Ducommun, Bernard ;

Scovassi, A. Ivana ;

Prosperi, Ennio .

NUCLEIC ACIDS RESEARCH, 2008, 36 (05) :1713-1722

[9] The F box protein S phase kinase-associated protein 2 regulates adipose mass and adipocyte number in vivo [J].

Cooke, Paul S. ;

Holsberger, Denise R. ;

Cimafranca, Melissa A. ;

Meling, Daryl D. ;

Beals, Charity M. ;

Nakayama, Keiko ;

Nakayama, Keiichi I. ;

Kiyokawa, Hiroaki .

OBESITY, 2007, 15 (06) :1400-1408

[10] Qualitative regional differences in adipose tissue growth and cellularity in male Wistar rats fed ad libitum [J].

DiGirolamo, M ;

Fine, JB ;

Tagra, K ;

Rossmanith, R .

AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 1998, 274 (05) :R1460-R1467

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