Identification of 14-3-3ε substrates from embryonic murine brain

被引：37

作者：

Ballif, Bryan A.

Cao, Zhongwei

Schwartz, Daniel

Carraway, Kermit L., III

Gygi, Steven P.

机构：

[1] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

[2] Univ Calif Davis, Ctr Canc, Sacramento, CA 95817 USA

来源：

JOURNAL OF PROTEOME RESEARCH | 2006年 / 5卷 / 09期

关键词：

phosphoproteomics; 14-3-3; epsilon; brain development; kinase; phosphorylation; mass spectrometry; deubiquitination;

D O I：

10.1021/pr060206k

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Mice deficient in 14-3-3 epsilon exhibit abnormal neuronal migration and die perinatally. We report here the first large-scale analysis of 14-3-3 interacting partners from primary animal tissue, identifying from embryonic murine brain 163 14-3-3 epsilon interacting proteins and 85 phosphorylation sites on these proteins. Phosphorylation of the deubiquitinating enzyme USP8 at serine 680 was found essential for its interaction with 14-3-3 epsilon and for maintaining USP8 in the cytosol.

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页码：2372 / 2379

页数：8

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