Polypeptides of hepatitis B surface antigen produced in transgenic potato

被引：33

作者：

Ehsani, P

Khabiri, A

Domansky, NN

机构：

[1] INST PASTEUR,DEPT MOL BIOL,TEHRAN 13164,IRAN

[2] INST PASTEUR,DEPT IMMUNOL,TEHRAN 13164,IRAN

来源：

GENE | 1997年 / 190卷 / 01期

关键词：

chimeric hepatitis B gene; middle protein; expression in plants;

D O I：

10.1016/S0378-1119(96)00647-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Chimeric genes containing the genomic fragments of hepatitis B virus (HBV) coding for middle (M) and major/small (S) surface proteins were constructed and expressed in transgenic potato plants under the control of the cauliflower mosaic virus 35S promoter. Enzyme-linked immunosorbent assay (ELISA) revealed the presence of HBV surface antigen (HBsAg) determinants in extracts from transgenic plants carrying both constructions, the pre-Sa determinant was identified using the corresponding monoclonal antibodies (mAb) in a dot immunobinding assay. Polypeptides of the immunoaffinity-purified S and M proteins were analyzed by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis. Two polypeptides of the M protein, probably representing different glycosylated forms, and the multimeric forms of HBsAg were observed.

引用

页码：107 / 111

页数：5

共 24 条

[1] IMMUNOAFFINITY PURIFICATION OF RECOMBINANT HEPATITIS-B SURFACE-ANTIGEN FROM YEAST USING A MONOCLONAL-ANTIBODY [J].