Assessment of hypoxia in experimental mice tumours by [18F]fluoromisonidazole PET and pO2 electrode measurements -: Infuence of tumour volume and carbogen breathing

被引:57
作者
Bentzen, L
Keiding, S
Horsman, MR
Grönroos, T
Hansen, SB
Overgaard, J
机构
[1] Aarhus Univ Hosp, Dept Expt Clin Oncol, Danish Canc Soc, DK-8000 Aarhus C, Denmark
[2] Aarhus Univ Hosp, PET Ctr, DK-8000 Aarhus C, Denmark
[3] Aarhus Univ Hosp, Dept Med, DK-8000 Aarhus C, Denmark
[4] Turku PET Ctr, Turku, Finland
关键词
D O I
10.1080/02841860260088863
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The aim of this study was to compare a non-invasive F-18-fluoromisonidazole ([F-18] FMISO) PET assessment of tumour hypoxia with invasive Eppendorf pO(2) measurements in 150-1500 mm(3) C3H mammary carcinomas transplanted on the back of CDF1 mice. The tumour-bearing mice breathed either carbogen gas (95% oxygen, 5% CO2) or normal air during bot h examinations. Additional autoradiography was performed in separate tumours treated similarly. The PET [F-18] FMISO examination significantly discriminated between tumours of carbogen and air-breathing mice. For the pO(2) measurements, there was a significantly lower percentage of measurements below 2.5 mmHg for carbogen-treated mice compared with air-breathing mice. However, no direct correlation between the methods was seen. A correlation was found between tumour volume and Eppendorf estimates of tumour hypoxia for the animals breathing normal air, but no correlation was found between the PET endpoint and tumour volume. This may be due to low pO(2) measurements obtained in necrotic tissue. Autoradiography confirmed lower [F-18] FMISO uptake in tumours of carbogen-breathing animals compared with air-breathing animals, and demonstrated the heterogeneity of the tracer uptake in small compared with larger tumours.
引用
收藏
页码:304 / 312
页数:9
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