Anti-biofilm efficacy of nitric oxide-releasing silica nanoparticles

被引：289

作者：

Hetrick, Evan M. ^{[1
]}

Shin, Jae Ho ^{[1
]}

Paul, Heather S. ^{[1
]}

Schoenfisch, Mark H. ^{[1
]}

机构：

[1] Univ N Carolina, Dept Chem, Chapel Hill, NC 27599 USA

来源：

BIOMATERIALS | 2009年 / 30卷 / 14期

基金：

美国国家卫生研究院;

关键词：

Nitric oxide; Nanoparticle; Biofilm; Antimicrobial; Cytotoxicity; POVIDONE-IODINE; ANAPHYLACTIC SHOCK; HYDROGEN-PEROXIDE; RESISTANCE; INFECTION; ANTIMICROBIALS; CHLORHEXIDINE; MICROBIOLOGY; ASSAY;

D O I：

10.1016/j.biomaterials.2009.01.052

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The ability of nitric oxide (NO)-releasing silica nanoparticles to kill biofilm-based microbial cells is reported. Biofilms of Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Staphylococcus epidermidis, and Candida albicans were formed in vitro and exposed to NO-releasing silica nanoparticles. Replicative viability experiments revealed that >= 99% of cells from each type of biofilm were killed via NO release, with the greatest efficacy (>= 99.999% killing) against gram-negative P. aeruginosa and E. coli biofilms. Cytotoxicity testing demonstrated that the highest dose of NO-releasing silica nanoparticles inhibited fibroblast proliferation to a lesser extent than clinical concentrations of currently administered antiseptics (e.g., chlorhexidine) with proven wound-healing benefits. This study demonstrates the promise of employing nanoparticles for delivering an antimicrobial agent to microbial biofilms (C) 2009 Elsevier Ltd. All rights reserved

引用

页码：2782 / 2789

页数：8

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