Histological markers for endothelial cells in endogenous and transplanted rodent pancreatic islets

被引：53

作者：

Mattsson, G ^{[1
]}

Carlsson, PO ^{[1
]}

Olausson, K ^{[1
]}

Jansson, L ^{[1
]}

机构：

[1] Uppsala Univ, Dept Med Cell Biol, Ctr Biomed, SE-75123 Uppsala, Sweden

来源：

PANCREATOLOGY | 2002年 / 2卷 / 02期

关键词：

lectin; Bandeiraea simplicifolia; pancreatic islets; microvasculature; endothelial cells; islet transplantation; revascularisation;

D O I：

10.1159/000055906

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

Background/Aims:To obtain a selective marker to identify endothelial cells is difficult, due to the heterogeneity of these cells. Most described markers perform well in some applications, but fail in others. The aim of this study was to identify a selective and specific marker for rodent microvascular endothelial cells, especially for use in studies on the vascular system of pancreatic islets. Methods: A biotin-labelled form of the lectin Bandeiraea or Griffonia simplicifolia in combination with a streptAB-Complex with alkaline phosphatase was used to stain endothelium in paraffin-embedded tissue sections from C57BL/6 mice, Sprague-Dawley or Wistar-Furth rats. Results: We were consistently able to selectively stain microvascular endothelial cells in lungs, small intestines, white and brown adipose tissue, pancreas and islets of Langerhans with the lectin Bandeiraea simplicifolia. Furthermore, we were able to visualise the vasculature in syngenically transplanted islets of Langerhans in Wistar-Furth rats and C57BL/6 mice. Attempts to stain rodent endothelial cells with antibodies against CD34, CD31, CD200, Ox43, von Willebrand factor and the lectin Ulex europaeus were not uniformly successful. Conclusion: The lectin Bandeiraea simplicifolia is a versatile marker for rodent endothelial cells, and may be used to study revascularisation after transplantation of pancreatic islet in rodents. Copyright (C) 2002 S. Karger AG, Basel and IAP.

引用

页码：155 / 162

页数：8

共 34 条

[1] LECTIN HISTOCHEMISTRY OF MAMMALIAN ENDOTHELIUM [J].

ALROY, J ;

GOYAL, V ;

SKUTELSKY, E .

HISTOCHEMISTRY, 1987, 86 (06) :603-607

[2] INTRAPORTALLY TRANSPLANTED PANCREATIC-ISLETS REVASCULARIZED FROM HEPATIC ARTERIAL SYSTEM [J].

ANDERSSON, A ;

KORSGREN, O ;

JANSSON, L .

DIABETES, 1989, 38 :192-195

[3]

BARCLAY AN, 1981, IMMUNOLOGY, V44, P727

[4] Capillary blood pressure in syngeneic rat islets transplanted under the renal capsule is similar to that of the implantation organ [J].

Carlsson, PO ;

Jansson, L ;

Andersson, A ;

Källskog, Ö .

DIABETES, 1998, 47 (10) :1586-1593

[5] PHENOTYPIC COMPARISON BETWEEN MESOTHELIAL AND MICROVASCULAR ENDOTHELIAL-CELL LINEAGES USING CONVENTIONAL ENDOTHELIAL-CELL MARKERS, CYTOSKELETAL PROTEIN MARKERS AND INVITRO ASSAYS OF ANGIOGENIC POTENTIAL [J].

CHUNGWELCH, N ;

PATTON, WF ;

YENPATTON, GPA ;

HECHTMAN, HB ;

SHEPRO, D .

DIFFERENTIATION, 1989, 42 (01) :44-53

[6] A general strategy for isolation of endothelial cells from murine tissues - Characterization of two endothelial cell lines from the murine lung and subcutaneous sponge implants [J].

Dong, QG ;

Bernasconi, S ;

Lostaglio, S ;

DeCalmanovici, RW ;

MartinPadura, I ;

Breviario, F ;

Garlanda, C ;

Ramponi, S ;

Mantovani, A ;

Vecchi, A .

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY, 1997, 17 (08) :1599-1604

[7]

DUIJVESTIJN AM, 1992, LAB INVEST, V66, P459

[8] Localization of the platelet-activating factor receptor to rat pancreatic microvascular endothelial cells [J].

Flickinger, BD ;

Olson, MS .

AMERICAN JOURNAL OF PATHOLOGY, 1999, 154 (05) :1353-1358

[9]

Garlanda C, 1997, EUR J CELL BIOL, V73, P368

[10] GRIFFONIA-SIMPLICIFOLIA .1. FLUORESCENT TRACER FOR MICROCIRCULATORY VESSELS IN NONPERFUSED THIN MUSCLES AND SECTIONED MUSCLE [J].

HANSENSMITH, FM ;

WATSON, L ;

LU, DY ;

GOLDSTEIN, I .

MICROVASCULAR RESEARCH, 1988, 36 (03) :199-215

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