Tumor necrosis factor alpha alters the cytotoxic effect of hydrogen peroxide in cultured hepatocytes

被引:20
作者
Imanishi, H [1 ]
Scales, WE [1 ]
Campbell, DA [1 ]
机构
[1] UNIV MICHIGAN,SCH MED,DEPT SURG,ANN ARBOR,MI 48109
关键词
D O I
10.1006/bbrc.1996.5901
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We examined whether tumor necrosis factor-alpha (TNF) affects the cytotoxic capacity of reactive oxygen species on rat hepatocytes in culture. Both TNF and reactive oxygen species are involved in many inflammatory events including hepatic ischemia/reperfusion injury and endotoxic shock. Synchronous treatment of hepatocytes with both TNF and H2O2 demonstrated that TNF (2000 ng/ml) enhanced the cytotoxic effect of H2O2 (500 mu M). By contrast, pretreatment with TNF (2000 ng/ml) for 24 h followed by exposure to H2O2 (1000 mu M) reduced the reactive oxygen-induced cytotoxicity. We conclude that TNF increases the effects of reactive oxygen-induced cytotoxicity when exposed synchronously, whereas TNF pretreatment induces a cytoprotective effect to reactive oxygen species, presumably by up-regulation of the reduced form of glutathione levels in hepatocytes. (C) 1997 Academic Press
引用
收藏
页码:120 / 124
页数:5
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