Direct action of nitric oxide on osteoblastic differentiation

被引：74

作者：

Hikiji, H

Shin, WS

Oida, S

Takato, T

Koizumi, T

Toyooka, T

机构：

[1] UNIV TOKYO,FAC MED,DEPT INTERNAL MED 2,TOKYO 113,JAPAN

[2] TOKYO MED & DENT UNIV,FAC DENT,DEPT ORAL BIOCHEM,BUNKYO KU,TOKYO 113,JAPAN

来源：

FEBS LETTERS | 1997年 / 410卷 / 2-3期

关键词：

NO; NOS gene expression; osteoblastic differentiation; TNF-alpha; IL-1; beta;

D O I：

10.1016/S0014-5793(97)00597-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The effect of nitric oxide (NO) on osteoblastic differentiation was examined in cultured mouse osteoblasts. Interleukin-1 beta and tumor necrosis factor-alpha expressed inducible NO synthase gene with little effect on constitutive NO synthase gene. These cytokines increased NO production, which was inhibited by L-NMMA pretreatment, and decreased alkaline phosphatase (AIPase) activity, which was not restored by L-NMMA. Furthermore, NO donors, sodium nitroprusside and NONOate dose-dependently elevated AIPase activity and expression of osteocalcin gene. These results suggest that NO directly facilitates osteoblastic differentiation and the cytokine-induced inhibition of AIPase activity is mediated via mechanism other than NO. (C) 1997 Federation of European Biochemical Societies.

引用

页码：238 / 242

页数：5

共 27 条

[1] APPARENT HYDROXYL RADICAL PRODUCTION BY PEROXYNITRITE - IMPLICATIONS FOR ENDOTHELIAL INJURY FROM NITRIC-OXIDE AND SUPEROXIDE [J].