Metabolic Engineering of the Tricarboxylic Acid Cycle for Improved Lysine Production by Corynebacterium glutamicum

被引：89

作者：

Becker, Judith ^{[1
]}

Klopprogge, Corinna ^{[2
]}

Schroeder, Hartwig ^{[2
]}

Wittmann, Christoph ^{[1
]}

机构：

[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Biochem Engn, D-38106 Braunschweig, Germany

[2] BASF SE, Res Fine Chem & Biotechnol, Ludwigshafen, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2009年 / 75卷 / 24期

关键词：

IN-VIVO; STRAIN IMPROVEMENT; FLUX; EXPRESSION; ASPARTOKINASE; DEHYDROGENASE; BIOSYNTHESIS; FRUCTOSE; PATHWAY; LEADS;

D O I：

10.1128/AEM.01942-09

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In the present work, lysine production by Corynebacterium glutamicum was improved by metabolic engineering of the tricarboxylic acid (TCA) cycle. The 70% decreased activity of isocitrate dehydrogenase, achieved by start codon exchange, resulted in a > 40% improved lysine production. By flux analysis, this could be correlated to a flux shift from the TCA cycle toward anaplerotic carboxylation.

引用

页码：7866 / 7869

页数：4

共 22 条

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