Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase

被引：23

作者：

Ruiz, C ^{[1
]}

Blanco, A ^{[1
]}

Pastor, FIJ ^{[1
]}

Diaz, P ^{[1
]}

机构：

[1] Univ Barcelona, Fac Biol, Dept Microbiol, Barcelona 08028, Spain

来源：

FEMS MICROBIOLOGY LETTERS | 2002年 / 217卷 / 02期

关键词：

Bacillus; Bacillus megaterium; lipase; carboxylesterase;

D O I：

10.1111/j.1574-6968.2002.tb11485.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The lipolytic system of Bacillus megaterium 370 was investigated, showing the existence of at least two secreted lipases and a cell-bound esterase. A gene coding for an extracellular lipase was isolated and cloned in Escherichia coli. The cloned enzyme displayed high activity on short to medium chain length (C-4-C-8) substrates, and poor activity on C-18 substrates. On the basis of amino acid sequence homology, the cloned lipase was classified into subfamily I.4 of bacterial lipases. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：263 / 267

页数：5

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